TU Rong-Han, TAN Fu-Lai, CHEN Hong-Kai, SU Yuan, TAO Yi-Xin, NI Da-Dan and LIAO De-Fu. Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province[J]. Virologica Sinica, 1989, 4(1).
Citation: TU Rong-Han, TAN Fu-Lai, CHEN Hong-Kai, SU Yuan, TAO Yi-Xin, NI Da-Dan, LIAO De-Fu. Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province .VIROLOGICA SINICA, 1989, 4(1) : 28.

安徽省流行性出血热病毒杂交瘤单克隆抗体的研制与应用

  • 用流行性出血热病毒(EHFV)李株和陈株血凝素(HAN)分别免疫Balb/C小鼠,取其脾细胞和骨髓瘤细胞SP2/O融合,杂交瘤生长孔率为97.5%和60%,阳性率各为2.5%和22%,克隆化培养阳性率达到100%。通过筛选得2株杂交瘤细胞、一株为2B_7、另一株为1H_8。其腹水单克隆抗体(McAb)滴度均达1∶16×10~4—32×10~4。1H_8的血凝抑制滴度达1∶5120。经染色体核型分析,杂交细胞染色体2B_7为80—97条,1H_8为85—105条,每个细胞均含一条中着丝点标记染色体。McAb的Ig类别和IgG亚类检测结果2B_7为IgM,1H_8为IgG_(2b)。两者对19株不同来源的EHFV具有不同的反应,1H_8McAb与19株都具有不同程度的免疫荧光反应,滴度都较高,且对5株不同来源的EHFV-HAN具有血凝抑制活性,滴度在1∶1280—5120,故1H_8为血凝抑制抗体;2B_7只对12株EHFV起反应,对7株不起反应,且没有血凝抑制活性。这说明1H_8与2B_7具有不同的特性。

Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province

  • Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...

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    Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province

    • 1. Division of Virclogy
    • 2. Anhui Institute of Medical Sciences

    Abstract: Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...

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