Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses

ZHOU Lv-Qian; XUE Cui-Feng; SUN Fu-Lin

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February 1989

ZHOU Lv-Qian, XUE Cui-Feng and SUN Fu-Lin. Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses[J]. Virologica Sinica, 1989, 4(1).

Citation: ZHOU Lv-Qian, XUE Cui-Feng, SUN Fu-Lin. Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses .VIROLOGICA SINICA, 1989, 4(1) : 109.

在琼脂双向扩散中植物病毒非特异带的产生和消除

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

检测植物病毒,通常采用琼脂双向扩散、对流免疫电泳和酶联免疫吸附测定(ELIsA)等三种方法,而在研究一些植物病毒之间的亲缘关系方面,琼脂双向扩散则能提供其他方法所不能提供的免疫学图谱。我们在做琼脂双向扩散时发现,植物病毒的某些寄主与提纯病毒时所用磷酸盐缓冲液之间常能形成非特异带。本文报道非特异带的干扰现象及其消除方法。
- 琼脂双扩散
- , 植物病毒
- , 非特异带

Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses

1.
Wuhan Institute of Virology. Academia Sinica
2.
Wuhan

Abstract

Phosphate is usually used in purifying plant viruses, but phosphate buffer can form unspecific bands with some hosts during purification by agar-double diffusion. Unspecific bands often interfere with the determination of specific bands in detection of the plant viruses. The absorbing method can be utilized to eliminate the interfering phenomenon.
- Agar-double diffusion
- , Plant viruses
- , Unspccific bands

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses

1. Wuhan Institute of Virology. Academia Sinica
2. Wuhan

Abstract: Phosphate is usually used in purifying plant viruses, but phosphate buffer can form unspecific bands with some hosts during purification by agar-double diffusion. Unspecific bands often interfere with the determination of specific bands in detection of the plant viruses. The absorbing method can be utilized to eliminate the interfering phenomenon.