Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs

TANG En-Hua; PENG Dun-Yang; WANG Li-Yan; LIU Ming-Ying; WEN Yu-Ling; RUAN Yong-Liang; XIE Tian-Hong; GUO Ren

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April 1989

TANG En-Hua, PENG Dun-Yang, WANG Li-Yan, LIU Ming-Ying, WEN Yu-Ling, RUAN Yong-Liang, XIE Tian-Hong and GUO Ren. Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs[J]. Virologica Sinica, 1989, 4(2).

Citation: TANG En-Hua, PENG Dun-Yang, WANG Li-Yan, LIU Ming-Ying, WEN Yu-Ling, RUAN Yong-Liang, XIE Tian-Hong, GUO Ren. Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs .VIROLOGICA SINICA, 1989, 4(2) : 135.

用免疫荧光单克隆抗体对脊髓灰质炎病毒抗原表位的分析

1.
中国医学科学院医学生物学研究所
2.
中国医学科学院医学生物学研究所昆明

摘要

用间接免疫荧光与中和试验筛选出来的抗脊髓灰质炎2型与3型不同毒株的12个单克隆抗体,其中3个仅有免疫荧光活性,9个具有中和与免疫荧光活性。用免疫荧光活性的单克隆抗体进行试验,发现它们在识别特异性抗原表位方面与中和性单克隆抗体相似,显示出株特异的、几个毒株共同特异的或型特异的抗原表位。根据表位分布关系及特征,可以用来鉴别型内毒株的特征、毒株的抗原分析、抗原变异的研究以及疫苗相关病例的鉴别。所得结果与中和性单克抗隆抗体及T1-寡核苷酸指纹图谱分析一致。而免疫荧光单克隆抗体识别抗原表位的活性范围比中和性单克隆抗体更广。另外还发现某些兼有荧光与中和两活性的同一个单克隆抗体,用不同方法(IF与NT)进行试验时,与相同毒株出现不同表位反应,这点是值得引起注意需待进一步证实的重要问题。
- 抗原表位
- , 免疫荧光单克隆抗体
- , 脊髓灰质炎病毒

Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs

1.
Institute of Medical Biology
2.
Chinese Academy of Medical Sciences

Abstract

12 McAbs to several strains of poliovirus Type 2 and 3 were selected by IF and NT, 9 McAbs had both neutralizing and immunofluorescence activity,3 McAbs were uniquely with IF staining activity. All the 12 McAbs with immunofluorescence activity reacted with poliovirus Type 2 and 3(F-McAbs) and were found similar to neutranizing McAbs (N-McAbs) recognizing different spesific antigenic epitopes on infected cells. According to topological relationships of antigenic epitopes, it was possible to characterize the ...
- Antigenic epitope
- , Immunofluorescence McAbs
- , Poliovirus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs

1. Institute of Medical Biology
2. Chinese Academy of Medical Sciences

Abstract: 12 McAbs to several strains of poliovirus Type 2 and 3 were selected by IF and NT, 9 McAbs had both neutralizing and immunofluorescence activity,3 McAbs were uniquely with IF staining activity. All the 12 McAbs with immunofluorescence activity reacted with poliovirus Type 2 and 3(F-McAbs) and were found similar to neutranizing McAbs (N-McAbs) recognizing different spesific antigenic epitopes on infected cells. According to topological relationships of antigenic epitopes, it was possible to characterize the ...