TANG Jia-Qi and LI Xian-Fu. Method for Preparation of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Antigens by Treatment Infected Mouse Brains and Lungs with Tween-ether[J]. Virologica Sinica, 1989, 4(2).
Citation: TANG Jia-Qi, LI Xian-Fu. Method for Preparation of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Antigens by Treatment Infected Mouse Brains and Lungs with Tween-ether .VIROLOGICA SINICA, 1989, 4(2) : 164.

肾综合征出血热病毒T-E抗原的制备及其特性研究

  • 本试验建立了用Tween 80和乙醚处理HFRSV感染鼠脑、肺制备特异性抗原的方法,该抗原具有安全、稳定、特异性好、制备简单的特点,可能是HFRSV抗原的共同组分。

Method for Preparation of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Antigens by Treatment Infected Mouse Brains and Lungs with Tween-ether

  • We first utilized infected mouse brains and lungs to prepare HFRSV specific antigens by treatment with Tween 80-ether. This procedure was very economical and easy. The T-E antigens was a more sensitive, uniform reagent for the RPHI or ELISA and non-multiplication. It also showed an increased thermal stablity at different temperatures and could be stored for more than one year at and below 4℃ without loss of antigenic activity.

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    Method for Preparation of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Antigens by Treatment Infected Mouse Brains and Lungs with Tween-ether

    • 1. Military Medical Research Institute
    • 2. Nanjing Command

    Abstract: We first utilized infected mouse brains and lungs to prepare HFRSV specific antigens by treatment with Tween 80-ether. This procedure was very economical and easy. The T-E antigens was a more sensitive, uniform reagent for the RPHI or ELISA and non-multiplication. It also showed an increased thermal stablity at different temperatures and could be stored for more than one year at and below 4℃ without loss of antigenic activity.

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