Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation

LIN Xiang-Dong; Ge-Chi-Hua; Tu-Chuan-Lin; HU Xin-Mei; ZHANG Hui-Xing; GAO Li; ZHOU Gong

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April 1989

LIN Xiang-Dong, Ge-Chi-Hua, Tu-Chuan-Lin, HU Xin-Mei, ZHANG Hui-Xing, GAO Li and ZHOU Gong. Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation[J]. Virologica Sinica, 1989, 4(2).

Citation: LIN Xiang-Dong, Ge-Chi-Hua, Tu-Chuan-Lin, HU Xin-Mei, ZHANG Hui-Xing, GAO Li, ZHOU Gong. Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation .VIROLOGICA SINICA, 1989, 4(2) : 208.

巨细胞病毒中性红斑定量试验与高滴度病毒的制备

1.
上海医科大学微生物学教研室
2.
云南省昭通地区防疫站
3.
上海职工医学院微生物教研室上海

摘要

在巨细胞病毒(CMV)的研究中常需对病毒定量。CMV需低滴度传代,否则会产生没有感染性的缺损病毒颗粒;CMV的抗原性受其感染量的影响;检测CMV中和抗体或纯化病毒都需具备病毒空斑定量基础。另外,制备高感染滴度的无细胞病毒(游离病毒)是对CMV进行分子生物学研究的前提。本文建立了CMV微量板法中性红斑定量技术并比较了几种制备无细胞CMV的方法。
- 人类巨细胞病毒
- , 空斑定量
- , 病毒制备
- , 高滴度病毒

Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation

1.
Department of Microbiology
2.
Shanghai Medical University
3.
Shanghai

Abstract

A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the application of the assay, With this assay, it was found that rotating culture of CMV-infected cells could easily produce infectious CMV titer as high as 1.8×10~7 PFU/ml, when the culture medium supernatant was co...
- Human cytomegalovirus
- , Plaque quantitation
- , Virus preparation
- , High titer virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation

1. Department of Microbiology
2. Shanghai Medical University
3. Shanghai

Abstract: A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the application of the assay, With this assay, it was found that rotating culture of CMV-infected cells could easily produce infectious CMV titer as high as 1.8×10~7 PFU/ml, when the culture medium supernatant was co...