LIU Hai-Lin. Cell Fusion by Epidemic Hemorrhagic Fever Virus[J]. Virologica Sinica, 1989, 4(2).
Citation: LIU Hai-Lin. Cell Fusion by Epidemic Hemorrhagic Fever Virus .VIROLOGICA SINICA, 1989, 4(2) : 216.

流行性出血热病毒的细胞融合作用

  • 我们的实验结果揭示流行性出血热病毒(EHFV)在酸性条件下可导致感染细胞发生融合,细胞间隙消失,细胞界限不清,细胞和细胞连在一起,形成多核的巨细胞体,姬姆薩染色比未融合细胞浅。融合液(Eagle’s基础培养液,含0.2%BSA,20mmol/L HEPES),用1.0NNaOH调pH至5.0—6.0时,细胞融合最甚,几乎所有的感染细胞都...

Cell Fusion by Epidemic Hemorrhagic Fever Virus

  • The results of our experimental research indicated that epidemic hemorrhagic feve virus (EHFV) couses cell to cell fusion under acidic conditions. Maximum fusions was obtained at pH5.0—6.0. The EHFV-infeeted cell was covered with medium overlaid by methylcellulose and thenthe cell culture developed the fusion foci. A linear relationship was observed between the relative virus concentration and the number of fusion foci. The result indicated that the titration of EHFV infectivity could be done by counting th...

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    Cell Fusion by Epidemic Hemorrhagic Fever Virus

    • 1. Wuhan Institute of Virology

    Abstract: The results of our experimental research indicated that epidemic hemorrhagic feve virus (EHFV) couses cell to cell fusion under acidic conditions. Maximum fusions was obtained at pH5.0—6.0. The EHFV-infeeted cell was covered with medium overlaid by methylcellulose and thenthe cell culture developed the fusion foci. A linear relationship was observed between the relative virus concentration and the number of fusion foci. The result indicated that the titration of EHFV infectivity could be done by counting th...

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