Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics

YAO Zhi-Qiang; YANG Wei-Song; LIU Jian; ZHANG Wen-Bin; BAI Xue-Fan; LI Xue-Rong; HUANG Mei

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June 1989

YAO Zhi-Qiang, YANG Wei-Song, LIU Jian, ZHANG Wen-Bin, BAI Xue-Fan, LI Xue-Rong and HUANG Mei. Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics[J]. Virologica Sinica, 1989, 4(3).

Citation: YAO Zhi-Qiang, YANG Wei-Song, LIU Jian, ZHANG Wen-Bin, BAI Xue-Fan, LI Xue-Rong, HUANG Mei. Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics .VIROLOGICA SINICA, 1989, 4(3) : 266.

流行性出血热病毒在人B淋巴母细胞中复制增殖

1.
第四军医大学唐都医院出血热室
2.
第四军医大学训练部电镜室
3.
第四军医大学训练部电镜室西安

摘要

本文应用细胞培养、免疫荧光及电镜技术研究了B淋巴母细胞对EHF病毒的易感性。结果表明EHF病毒可在该细胞中增殖。感染细胞无明显细胞病变,在形态上与对照组无差别。虽然大部分感染细胞呈现明亮病毒抗原荧光,但在电镜下却难以找到完整的病毒颗粒,仅在扩张的囊泡中发现一些性质待定的微丝样物质。人B淋巴母细胞持续感染的建立,提示患者外周血中大量出现的异型淋巴细胞可能允许EHF病毒在其中复制。
- 流行性出血热
- , 病毒定位
- , B淋巴母细胞

Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics

1.
Lab of HFRS/Inf Dis Dept
2.
Second Affiliated Hosp
3.
Fourth Millitary Med College

Abstract

The susceptibility of human B lymphoblastoid cells to HFRS virus infectionwas studied. The results showed that the cells were productively infected byHFRS virus. The persistently infected cells had no obvious cytopathic effects andwere indistinguishable morphologically from uninfected cells. It was difficult toidentify complete virions in infected lymphoblasts by electron microscopy con-trasted with strong immunofluorescence staining for intracellular HFRS virusantigens observed in the majority of these cel...
- epidemic hemorrhagic fever virus
- , human B lymphoblasts

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics

1. Lab of HFRS/Inf Dis Dept
2. Second Affiliated Hosp
3. Fourth Millitary Med College

Abstract: The susceptibility of human B lymphoblastoid cells to HFRS virus infectionwas studied. The results showed that the cells were productively infected byHFRS virus. The persistently infected cells had no obvious cytopathic effects andwere indistinguishable morphologically from uninfected cells. It was difficult toidentify complete virions in infected lymphoblasts by electron microscopy con-trasted with strong immunofluorescence staining for intracellular HFRS virusantigens observed in the majority of these cel...