The Detection of Potato Leaf--roll Virus by the Microslide Gel Double Diffusion Test
Abstract: The microslide gel double diffusion test was used to detect potato leaf-rollvirus (PLRV). It was found to be sufficiently sensitive for the detection ofPLRV in extracts of infected plants. A 1: 4 dilution of extract of PLRV infectedpotato stem could be detected unambiguously. The sensitivity of microslidewas compared with that of the Ouchterlony plate technique. The microslide te-chnique was capable of detecting 1 μg/ml of purified PLRV. This sensitivitywas sixfold as much as that of the Ouchterlony plate t...