Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

CHEN Huo-Sheng; GUO Hui-Yu; LUO Jin-Xian; HU Jin-Xin

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August 1989

CHEN Huo-Sheng, GUO Hui-Yu, LUO Jin-Xian and HU Jin-Xin. Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization[J]. Virologica Sinica, 1989, 4(4).

Citation: CHEN Huo-Sheng, GUO Hui-Yu, LUO Jin-Xian, HU Jin-Xin. Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization .VIROLOGICA SINICA, 1989, 4(4) : 365.

应用DNA-RNA斑点杂交法检测登革病毒核酸

1.
中山医科大学微生物学与免疫学教研室
2.
中山大学生物工程研究中心
3.
中山大学生物工程研究中心广州

摘要

新近制备了大量纯化的pEH920 DNA,该质粒DNA插入了登革病毒2型核酸片段的互补DNA。以[a-~(32)P]dCTP按缺口转译法标记pEH 920 DNA作为探针,以感染病毒的蚊细胞c_6/36培养上清作标本,应用DNA-RNA斑点杂交法检测了登革病毒核酸。结果显示同位素标记探针(pEH 920)与登革病毒2型标本反应最强,具有一定的型特异性。但与其它血清型登革病毒也呈一定交叉反应。初步探讨了探针的敏感性,至少可检出TCID_(50)625的登革病毒2型核酸。
- 登革病毒
- , 缺口转译
- , 斑点杂交

Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

1.
Department of Microbiology and Immunology
2.
Sun Yat-sen University of Medical Sciences
3.
GuangzhouBiotechnology Research Center

Abstract

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...
- Dengue virus
- , Nick translation
- , Dot-spot hybridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

1. Department of Microbiology and Immunology
2. Sun Yat-sen University of Medical Sciences
3. GuangzhouBiotechnology Research Center

Abstract: Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...