The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

PENG Hai; SHEN Xue-Ren; WU Jian-Hua; GONG Zu-Xun

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August 1989

PENG Hai, SHEN Xue-Ren, WU Jian-Hua and GONG Zu-Xun. The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro[J]. Virologica Sinica, 1989, 4(4).

Citation: PENG Hai, SHEN Xue-Ren, WU Jian-Hua, GONG Zu-Xun. The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro .VIROLOGICA SINICA, 1989, 4(4) : 427.

与TMV-RNA装配起始位点互补的cDNA片段对TMV体外装配的抑制

1.
中国科学院上海生物化学研究所
2.
中国科学院上海生物化学研究所上海

摘要

合成了与TMV-RNA病毒装配起始位点互补的、长度为二十个核苷酸的DNA片段。该片段用~(32)P标记后,代替反义RNA(antisense RNA)与TMV-RNA进行硝基纤维素膜点杂交和溶液杂交。结果表明,该cDNA片段在两种条件下均能与TMV-RNA进行杂交。将溶液杂交的RNA-cDNA复合体经酒精沉淀,再与TMV衣壳蛋白的20S聚合体制剂进行体外装配,用测定310nm吸收光谱变化和电子显微镜观察的方法鉴定装配结果。实验证明,该cDNA片段与TMV-RNA杂交后抑制了装配起始位点的活力,从而使TMV病毒颗粒的装配不能完成。这一结果提示,TMV基因装配起始位点顺宁的cDNA和反义RNA能够在体外抑制TMV病毒颗粒的装配。
- 烟草花叶病毒
- , cDNA片段
- , 杂交
- , 体外装配

The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

1.
Shanghai Institute of Biochemistry
2.
Academia Sinica. Shanghai

Abstract

A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...
- Tobacco mosaic virus （TMV）
- , cDNA fragment Hybridization
- , In vitro assembly

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

1. Shanghai Institute of Biochemistry
2. Academia Sinica. Shanghai

Abstract: A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...