Study on Bean Yellow Mosaic Virus Isolated from Alfalfa

ZHANG Xiang-Lin; YIN Yu-Qi; LI Guo-Ying; CUI Xing-Ming

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February 1990

ZHANG Xiang-Lin, YIN Yu-Qi, LI Guo-Ying and CUI Xing-Ming. Study on Bean Yellow Mosaic Virus Isolated from Alfalfa[J]. Virologica Sinica, 1990, 5(1).

Citation: ZHANG Xiang-Lin, YIN Yu-Qi, LI Guo-Ying, CUI Xing-Ming. Study on Bean Yellow Mosaic Virus Isolated from Alfalfa .VIROLOGICA SINICA, 1990, 5(1) : 88.

菜豆黄色花叶病毒(BYMV)—新疆苜蓿分离株的鉴定

1.
石河子农学院植病教研室
2.
石河子农学院植病教研室新疆石河子
3.
新疆石河子

摘要

从新疆苜蓿黄斑花叶病株上分离到病毒分离物M-4,该分离物能引起多种豆科值物系统花叶,并在藜科植物上产生局部褪绿斑,易经汁液摩擦接种和蚜虫传毒,不经菜豆种子传毒。病毒致死温度60—65℃,体外保毒期4—5天,稀释限点10~(-3)—10~(-4)。病毒粒体线状,长约660—740nm,宽15nm;在感病的寄主叶片细胞中,电镜观察到风轮状、带状和环状内含体。免疫电镜法测定,该分离物与菜豆黄色花叶病毒(BYMV)抗血清有血清反应。经SDS-聚丙烯酰胺凝胶电泳和氨基酸自动分析仪分析分别测得该病毒的衣壳蛋白亚基分子量为16,200道尔顿,氨基酸残基数128个。鉴定结果认为,分离物M-4是BYMV的一个株系。
- 菜豆黄色花叶病毒
- , 苜蓿分离株

Study on Bean Yellow Mosaic Virus Isolated from Alfalfa

1.
Laboratory of plant pathology
2.
Shihezi Agricultural College
3.
Shihezi

Abstract

A virus isolate M-4 is obtained from alfalfa plant with yellow spot mosaic in Xingjiang. The M-4 isolate could cause systematic mosaic on many kinds of Leguminosae plants and give local chlorosis spots on Chenopodiaceae plants The virus is easily to be transmitted by sap and aphis inoculation, but not by bean seeds, The thermal death point is60—65℃; the dilution end point is 10~(-3)—10~(-4) and the longevity in vitro is 4—5 days The size of the filament particles is about 660—740×15nm in purified virus prep...
- Bean Yellow Mosaic Virus
- , Alfalfa s isolate

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on Bean Yellow Mosaic Virus Isolated from Alfalfa

1. Laboratory of plant pathology
2. Shihezi Agricultural College
3. Shihezi

Abstract: A virus isolate M-4 is obtained from alfalfa plant with yellow spot mosaic in Xingjiang. The M-4 isolate could cause systematic mosaic on many kinds of Leguminosae plants and give local chlorosis spots on Chenopodiaceae plants The virus is easily to be transmitted by sap and aphis inoculation, but not by bean seeds, The thermal death point is60—65℃; the dilution end point is 10~(-3)—10~(-4) and the longevity in vitro is 4—5 days The size of the filament particles is about 660—740×15nm in purified virus prep...