Study on the Propagation of Duck and Gosling Plague Viruses in identical Host System

HONG Feng; HUANG Yin-Xian

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February 1990

HONG Feng and HUANG Yin-Xian. Study on the Propagation of Duck and Gosling Plague Viruses in identical Host System[J]. Virologica Sinica, 1990, 5(1).

Citation: HONG Feng, HUANG Yin-Xian. Study on the Propagation of Duck and Gosling Plague Viruses in identical Host System .VIROLOGICA SINICA, 1990, 5(1) : 102.

鹅源鸭瘟病毒和小鹅瘟病毒在同一宿主系统增殖的观察

洪锋 ,
黄引贤

1.
华南农业大学兽医系
2.
华南农业大学兽医系广州

摘要

本文首次报道了鹅源鸭瘟病毒(DPV—Ⅰ)和鸭胚化小鹅瘟病毒(GPV—Ⅰ)能同时在同一鸭胚内复制增殖,未发现干扰作用,在理论上说明某些不相关的两种病毒可在同一宿主增殖,实践上为利用同一鸭胚研制二联疫苗提供了依据。研究结果表明:1.DPV—Ⅰ和GPV—Ⅰ联合感染同一鸭胚后,其尿囊液在电镜下见两种病毒,DPV—Ⅰ呈园形或椭园形,有囊膜,直径为38—109nm,GPV-Ⅰ呈园形,无囊膜,直径为18—25nm;2.含毒尿囊液使鸭胚成纤维细胞(DEF)单层发生细胞病变作用(CPE),证实存在DPV-Ⅰ,而用小鹅瘟微量免疫扩散(MID)试验,又能检出GPV-Ⅰ抗原;3.含毒尿囊液免疫鹅的血清中存在抗两种病毒的(?)和抗体和GPV沉淀抗体;4.含毒尿囊液免疫的成鹅对DPV强毒攻击有相当免疫力,免疫鹅血清能中和GPV,使其失去对鸭胚的致病力;5.GPV-Ⅰ单独或与DPV-Ⅰ联合感染DEF单层后,均未见在细胞上复制。
- 鹅源鸭瘟病毒
- , 小鹅瘟病毒
- , 联合感染

Study on the Propagation of Duck and Gosling Plague Viruses in identical Host System

1.
South China Agricultural University
2.
Guangzhou

Abstract

It is the first time in the present paper to report the propagation of duck plague virus (DPV-Ⅰ) and gosling plagae virus (GPV-Ⅰ) in the identical duck embryo, what have been revealed in the results are as follows: 1. Both DPV and GPV particles were detected under electronic microscope in the CAF of the duck embryoes infected with DPV-Ⅰ and GPV-Ⅰ. 2. CAF with viruses could produce eytopathic effect (CPE)in the duck embryonic blast (DEF)cell monolayers, showing the existence of DPV; and GPV antigens were det...
- Co-infection DPV GPV

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Propagation of Duck and Gosling Plague Viruses in identical Host System

1. South China Agricultural University
2. Guangzhou

Abstract: It is the first time in the present paper to report the propagation of duck plague virus (DPV-Ⅰ) and gosling plagae virus (GPV-Ⅰ) in the identical duck embryo, what have been revealed in the results are as follows: 1. Both DPV and GPV particles were detected under electronic microscope in the CAF of the duck embryoes infected with DPV-Ⅰ and GPV-Ⅰ. 2. CAF with viruses could produce eytopathic effect (CPE)in the duck embryonic blast (DEF)cell monolayers, showing the existence of DPV; and GPV antigens were det...