Application of Plaque Assay Method for Rabies Virus in the Study of Virus and Vaccine

LI Hong-Ling; LI He-Min; YU Yong-Xin; YAN Zi-Lin

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April 1990

LI Hong-Ling, LI He-Min, YU Yong-Xin and YAN Zi-Lin. Application of Plaque Assay Method for Rabies Virus in the Study of Virus and Vaccine[J]. Virologica Sinica, 1990, 5(2).

Citation: LI Hong-Ling, LI He-Min, YU Yong-Xin, YAN Zi-Lin. Application of Plaque Assay Method for Rabies Virus in the Study of Virus and Vaccine .VIROLOGICA SINICA, 1990, 5(2) : 135.

狂犬病毒蚀斑方法在病毒研究和疫苗研制中的应用

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京

摘要

本文采用狂犬病毒CTN-1和4aC株,经Vero细胞传代适应后,以Vero细胞为培养基质,建立了狂犬病毒蚀斑试验和蚀斑减少试验的方法。目前已将此方法应用于病毒鉴定、病毒克隆、病毒滴定以及抗狂犬血清的检测,并取得了较好的结果。
- 狂犬病毒
- , 蚀斑方法

Application of Plaque Assay Method for Rabies Virus in the Study of Virus and Vaccine

1.
National Institute for the Control of Pharmaceutical and Biological Products. Beijing

Abstract

We explored rabies virus adapted to Vero cells making plaque formation promptly.The plaque could be all exposed and easily seen under microscope on 4th day. It was shown that plaque method was more sensitive than mice(i.c) method on the titer of virus and assay neutralizing antibody.Up to now, the plaque formation has been applied to virus cloning, virus identify, virus titration,evaluation of serum-antibody content by the virus neutralization technique,providing an important assay method for the study and ...
- Rabies virus
- , Plaque method

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Application of Plaque Assay Method for Rabies Virus in the Study of Virus and Vaccine

1. National Institute for the Control of Pharmaceutical and Biological Products. Beijing

Abstract: We explored rabies virus adapted to Vero cells making plaque formation promptly.The plaque could be all exposed and easily seen under microscope on 4th day. It was shown that plaque method was more sensitive than mice(i.c) method on the titer of virus and assay neutralizing antibody.Up to now, the plaque formation has been applied to virus cloning, virus identify, virus titration,evaluation of serum-antibody content by the virus neutralization technique,providing an important assay method for the study and ...