Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus

WANG Xun-Zhang; XIE Wei-Dong; HE Dai-Fen; ZHENG Xiao-Wen; LONG Qi-Xin; PANG Yi; PU Zhe-Long

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April 1990

WANG Xun-Zhang, XIE Wei-Dong, HE Dai-Fen, ZHENG Xiao-Wen, LONG Qi-Xin, PANG Yi and PU Zhe-Long. Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus[J]. Virologica Sinica, 1990, 5(2).

Citation: WANG Xun-Zhang, XIE Wei-Dong, HE Dai-Fen, ZHENG Xiao-Wen, LONG Qi-Xin, PANG Yi, PU Zhe-Long. Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1990, 5(2) : 167.

粉纹夜蛾核型多角体病毒诱导的胸苷激酶特性研究

1.
中山大学昆虫学研究所
2.
中山大学生物化学专业
3.
中山大学昆虫学研究所广州
4.
届本科毕业生

摘要

粉纹夜蛾(Trichoplusia ni)核型多角体病毒(TnNPV)感染草地贪夜蛾(Spodop-tera frugiperda)细胞后能诱导提高胸苷激酶的活性。不论是正常或感染细胞中的胸苷激酶都可将脱氧胞苷磷酸化,酶活最适pH及Mg~(++)离子浓度值基本相同,DEAE-纤维素和Cibacron blue-sepharose柱层析所得酶活性图谱亦相似。TnNPV在胸苷激酶缺陷型的草地贪夜蛾细胞中能正常复制,但被感染细胞不具有胸苷激酶活性。由此可以确定,经TnNPV感染诱导后,活性有所提高的胸苷激酶不是病毒基因编码的。
- 杆状病毒
- , 粉纹夜蛾
- , 胸苷激酶
- , 缺陷型细胞

Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus

1.
Institute of Entomology
2.
Zhongshan University
3.
Guangzhou 510275

Abstract

Trichoplusia ni nuclear polyhedrosis virus(TnNPV) stimulated thymidine kinase(TK) activities in infected Spodoptera frugiperda cells. TK activities both in infected and uninfected cells are similar in their pH, Mg~(++) optima for assays and elution patterns on DEAE-cellulose and Cibacron-blue sepharose chromatography. The facts that TnNPV could replicate in TK~- S. frugiperda cells but no TK activity was detected in the infected cells implies that the virus-stimulated activity was not contributed by the vir...
- Baculovirus
- , Trichoplusia ni
- , Thymidine
- , Kinase（TK）

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus

1. Institute of Entomology
2. Zhongshan University
3. Guangzhou 510275

Abstract: Trichoplusia ni nuclear polyhedrosis virus(TnNPV) stimulated thymidine kinase(TK) activities in infected Spodoptera frugiperda cells. TK activities both in infected and uninfected cells are similar in their pH, Mg~(++) optima for assays and elution patterns on DEAE-cellulose and Cibacron-blue sepharose chromatography. The facts that TnNPV could replicate in TK~- S. frugiperda cells but no TK activity was detected in the infected cells implies that the virus-stimulated activity was not contributed by the vir...