Comparative Study of the Fingerprinting Map of Granulins and Polyhedrins

ZHOU Xiao-Feng; Ye Lin-Bai; Liu Nian-Cui

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April 1990

ZHOU Xiao-Feng, Ye Lin-Bai and Liu Nian-Cui. Comparative Study of the Fingerprinting Map of Granulins and Polyhedrins[J]. Virologica Sinica, 1990, 5(2).

Citation: ZHOU Xiao-Feng, Ye Lin-Bai, Liu Nian-Cui. Comparative Study of the Fingerprinting Map of Granulins and Polyhedrins .VIROLOGICA SINICA, 1990, 5(2) : 175.

几株杆状病毒包涵体蛋白双向高压电泳指纹图谱比较

1.
武汉大学病毒系
2.
武汉大学病毒系武汉

摘要

用蔗糖密度梯度离心提纯的6种昆虫病毒包涵体(BsNPV1,BsNPV2,BtNPV,EpNPV,PrGV,PxGV)经DAS碱解,粗提纯的包涵体蛋白经Sepharose-6B柱层析和Caaalco-prep-disc法进一步纯化后,在SDS-PAGE中显示为一条分子量约28,000d的带。用常规双向免疫扩散法检查各种包涵体蛋白之间的差异,结果表明血清学BsNPV与BtNPV,PrGV与PxGV之间都是不可分辩的。6种包涵体蛋白的双向高压指纹图谱表明,它们的指纹图谱都不同程度地存在着一些相同或十分相似的肽点。就每种病毒的指纹图谱又都是独特的,NPV间相似性低于GV间相似性。我们认为NPV和GV的包涵体蛋白在结构上存在着一些相同的或相似的保守区域,但不同种之间在整个一级结构上是有差异的。利用包涵体蛋白的指纹图谱鉴定杆状病毒是个灵敏可行的方法。
- 包涵体蛋白
- , 指纹图谱
- , 双向扩散

Comparative Study of the Fingerprinting Map of Granulins and Polyhedrins

1.
Dept. of Viroloqy
2.
Wuhan University

Abstract

Six kinds of baculoviruses(BsNPV1, BsNPV2, BtNPV, EpNPV, PrGV, PxGV) are purified by centrifugation using sucrose gradient,Roughly purified granulins and polyhedrins are obtained by having the inclusion body solubilized in dilute alkaline salne after possible alkaline protease activity is inhibited. Then granulins and polyhedrins in highly pure form are obtined by using Sepharose-6B chromatography and SDS-polyacrylamide prep-disc(Canalco). Each kind of granulins and polyhedrins shows only one band on SDS-PA...
- Nuclear polyhedrin
- , Fingerprint
- , Double diffusion

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparative Study of the Fingerprinting Map of Granulins and Polyhedrins

1. Dept. of Viroloqy
2. Wuhan University

Abstract: Six kinds of baculoviruses(BsNPV1, BsNPV2, BtNPV, EpNPV, PrGV, PxGV) are purified by centrifugation using sucrose gradient,Roughly purified granulins and polyhedrins are obtained by having the inclusion body solubilized in dilute alkaline salne after possible alkaline protease activity is inhibited. Then granulins and polyhedrins in highly pure form are obtined by using Sepharose-6B chromatography and SDS-polyacrylamide prep-disc(Canalco). Each kind of granulins and polyhedrins shows only one band on SDS-PA...