ZHANG De-Li. Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty[J]. Virologica Sinica, 1990, 5(2).
Citation: ZHANG De-Li. Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty .VIROLOGICA SINICA, 1990, 5(2) : 221.

敏感性不同细胞系交替选育获得超高增殖率细小病毒株

  • 迄今为止未见国际上有组织培养水貂肠炎病毒(MEV)、猫泛白细胞减少症病毒(FPLV)、犬细小病毒(CPV)的TCID_(50)高于log_(10)7.0或MEV的HA高于32~或CPV的HA高于4096~的报道。又据Goto(1986)报道,TCID_(50)为log10~(4-6)的CPV比MEV。FPLV的HA高20多倍,故HA为4096~的CPV液比HA为32~的MEV液中病毒含量还低。由此可见,PV培养效价低是目前国际上急待解决的重大问题。

Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty

  • This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) strain and insusceptibility C_(81) strain ever reported as yet. The systematic tests proved that SMEV_(18,36) strains selected at present by alternative cultivation are super reproductive with powerful and extens...

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    Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty

    • 1. Economic Animal Virology and Immunology Laboratory

    Abstract: This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) strain and insusceptibility C_(81) strain ever reported as yet. The systematic tests proved that SMEV_(18,36) strains selected at present by alternative cultivation are super reproductive with powerful and extens...

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