The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It

ZHANG Ming; CHEN Shen-Bao; DING Ru-Ning; ZHOU Yao-Xi

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June 1990

ZHANG Ming, CHEN Shen-Bao, DING Ru-Ning and ZHOU Yao-Xi. The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It[J]. Virologica Sinica, 1990, 5(3).

Citation: ZHANG Ming, CHEN Shen-Bao, DING Ru-Ning, ZHOU Yao-Xi. The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It .VIROLOGICA SINICA, 1990, 5(3) : 278.

Raji细胞培养中HSV持续感染模型的建立及免疫因素(TNF IFNs)对它的影响

1.
南京医学院微生物学教研室
2.
南京医学院微生物学教研室南京

摘要

本文观察HSV持续感染Raji细胞培养物150天。发现整个感染过程似呵分为两个阶段:急性期(前30天)和稳定期(30天以后)。在急性期上清液中HSV滴度达10~(6.2)TCID_(50)/ml.病毒抗原阳性细胞达21％,死细胞达42％;在稳定期病毒和细胞处于相对平衡状态,上清液中IISV滴度在10~(3.5-4.2)TCID_(50)/ml,病毒抗原阳性细胞约占5—10％,感染细胞与对照细胞在生长特性上无明显差异。用rIFNs、rlL-2和TNF处理稳定期的细胞培养物,发现TNF和rlFNa能明显抑制HSV的复制,rIENy作用较弱,去除上述因子5天后又恢复到处理前水平。rlL-2无明显作用。用HSV抗体处理上述细胞培养物上清液中病毒和病毒抗原阳性细胞都消失,且在去除抗体后连续观察50天仍未出现。本实验为体外研究HSV持续感染提供了一个有用的模型。
- 单纯疱疹病毒
- , Raji细胞持续感染
- , 肿瘤坏死因子
- , 干扰素
- , 抗体

The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It

1.
Department of Microbiology
2.
Nanjing Medical College

Abstract

Persistent infection of herpes simplex virus(HSV) had been maintained in human B lymphoblastoid(Raji) cells for 150 days. The whole course of the persistent infection can be divided into two phases: acute(within 30 days p. i.) and persistent stable(30 days later p.i.). In the acute phase,the virus in the supernatent reached 10~(6?2)TCID_(50)/ml, HSV antigen positive cells(HSV Ag~+ cell) detected by immunofluorescence reached 21% and the percentage of dead cells reached 42%. In the stable phase, the virus an...
- HSV Raji cell Persistent infection TNF IFN Antibody

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It

1. Department of Microbiology
2. Nanjing Medical College

Abstract: Persistent infection of herpes simplex virus(HSV) had been maintained in human B lymphoblastoid(Raji) cells for 150 days. The whole course of the persistent infection can be divided into two phases: acute(within 30 days p. i.) and persistent stable(30 days later p.i.). In the acute phase,the virus in the supernatent reached 10~(6?2)TCID_(50)/ml, HSV antigen positive cells(HSV Ag~+ cell) detected by immunofluorescence reached 21% and the percentage of dead cells reached 42%. In the stable phase, the virus an...