Detection of Plant Viruses by Dot-immunobinding Assay

ZHOU Xue-Ping; PU Jie-Qin

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Volume 05 Issue 3

June 1990

ZHOU Xue-Ping and PU Jie-Qin. Detection of Plant Viruses by Dot-immunobinding Assay[J]. Virologica Sinica, 1990, 5(3).

Citation: ZHOU Xue-Ping, PU Jie-Qin. Detection of Plant Viruses by Dot-immunobinding Assay .VIROLOGICA SINICA, 1990, 5(3) : 317.

应用斑点法检测植物病毒的研究

周雪平 ,
濮祖芹

1.
南京农业大学植物病理教研组
2.
南京农业大学植物病理教研组南京

摘要

应用斑点法检测了病叶粗汁液中的芜菁花叶病毒(TuMV)、大豆花叶病毒(sMV)和黄瓜花叶病毒(CMV),病叶粗汁液可被检测的最大稀释度分别为1:5120、1:2560和1:1280。提纯的大豆花叶病毒和黄瓜花叶病毒可检测的最低限量分别为1.7ng和1.2ng。以牛血清白蛋白、吐温和聚乙烯吡咯啉酮作封闭液,均可获得满意的结果。应用斑点法检测芜菁花叶病毒和大豆花叶病毒时,其抗血清稀释1:500倍可获得满意效果,稀释2000倍仍可用于检测。
- 斑点法
- , 植物病毒检测
- , 芜菁花叶病毒
- , 大豆花叶病毒
- , 黄瓜花叶病毒

Detection of Plant Viruses by Dot-immunobinding Assay

1.
Section of Plant Pathology
2.
Nanjimg Agricultural University

Abstract

TuMV, SMV, and CMV were detected by dot-immunobinding assay (DIA) at dilutions of 1:5120, 1:2560 and 1:1280, respectively. DIA successfully detected 1.7ng of purified SMV and 1.2ng of purified CMV. DIA was used with horseradish peroxidase-linked goat anti-rabbit IgG and nitrocellulose membrane was used as the solid phase. 3, 3-Diamino-benzidine was used as the suhstrate and got good results. Absorption of the first antibody with healthy plant sap was necessary to eliminate background color. This assay is si...
- Dot-immunobinding assay Plant virus detection Soybean mosaic virus Turnip mosaic virus Cucumber mosaic virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Plant Viruses by Dot-immunobinding Assay

1. Section of Plant Pathology
2. Nanjimg Agricultural University

Abstract: TuMV, SMV, and CMV were detected by dot-immunobinding assay (DIA) at dilutions of 1:5120, 1:2560 and 1:1280, respectively. DIA successfully detected 1.7ng of purified SMV and 1.2ng of purified CMV. DIA was used with horseradish peroxidase-linked goat anti-rabbit IgG and nitrocellulose membrane was used as the solid phase. 3, 3-Diamino-benzidine was used as the suhstrate and got good results. Absorption of the first antibody with healthy plant sap was necessary to eliminate background color. This assay is si...