Development of EHF Virus Semi-micro Methylcellulose Plaque Assay

NIE Zi-Lin; YU Yong-Xin; YAO Zhi-Hui

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August 1990

NIE Zi-Lin, YU Yong-Xin and YAO Zhi-Hui. Development of EHF Virus Semi-micro Methylcellulose Plaque Assay[J]. Virologica Sinica, 1990, 5(4).

Citation: NIE Zi-Lin, YU Yong-Xin, YAO Zhi-Hui. Development of EHF Virus Semi-micro Methylcellulose Plaque Assay .VIROLOGICA SINICA, 1990, 5(4) : 357.

流行性出血热(EHF)病毒半微量甲基纤维素空斑法的建立

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京

摘要

本文报道EHF病毒一种新的空斑形成按术,即半微量甲基纤维素空斑法的建立及其应用。6株来源不同的毒株均可在V_(ero)-E_6细胞或V_(ero)细胞上形成清晰的空斑,形成的空斑能被特异抗EHF病毒血清所中和。其敏感性与用琼脂糖作覆盖物的空斑法一致。该法具有操作简便、快速等优点,适用于EHF的病原学和血清学研究。
- 流行性出血热病毒
- , 甲基纤维素
- , 半微量空斑法
- , Vero-E_6细胞
- , Vero细胞
- , 中和试验

Development of EHF Virus Semi-micro Methylcellulose Plaque Assay

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
Beijing 100050

Abstract

A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...
- Epidemic hemorrhagic fever （EHF） virus
- , Methylcellulose
- , Semi-micro plaque assay
- , Vero-E_（?） cells
- , Vero cells
- , Neutralization test

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Development of EHF Virus Semi-micro Methylcellulose Plaque Assay

1. National Institute for the Control of Pharmaceutical and Biological Products
2. Beijing 100050

Abstract: A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...