Nuclear Polyhedrosis Virus from Ectropis obliqua hypulina Wehrli in its Host Larvae

XIE Gong-Yin; HU Cui

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August 1990

XIE Gong-Yin and HU Cui. Nuclear Polyhedrosis Virus from Ectropis obliqua hypulina Wehrli in its Host Larvae[J]. Virologica Sinica, 1990, 5(4).

Citation: XIE Gong-Yin, HU Cui. Nuclear Polyhedrosis Virus from Ectropis obliqua hypulina Wehrli in its Host Larvae .VIROLOGICA SINICA, 1990, 5(4) : 397.

温度对茶尺蠖核型多角体病毒增殖动态的影响

叶恭银 ,
胡萃

1.
浙江农业大学植保系
2.
浙江农业大学植保系杭州

摘要

多角体计数、对流免疫电泳、单向免疫扩散及火箭免疫电泳测定的结果表明:26℃适于茶尺蠖核型多角体病毒(EoNPV)的增殖。多角体含量或其相对值随着时间的推移而增长,并渐趋于平稳,两者间呈Logistic曲线关系。单位体重或单头幼虫所含的多角体数量(y_1或y_2)、扩散环直径(y_3)和火箭峰值(y_4)与时间(t)的关系式分别为:y_1=(8.1481)/(1+EXP(9.4210-0.0608t))×10~9PIB/克;y_2=(6.1596)/(1+EXP(5.4809-0.0376t))×10~8PIB/头,y_3=(1.4)/(1+EXP(2.710-0.015t))cm;y_4=(3.52)/(1+EXP(4.580-0.040t))cm。但30℃下,EoNPV增殖严重受抑制,饲毒后24～168小时内难以测出多角体,其后多角体含量也极显著低于26℃,乃至难以被三种免疫测定法测出。
- 茶尺蠖
- , 核型多角体病毒
- , 温度对病毒增殖动态的影响

Nuclear Polyhedrosis Virus from Ectropis obliqua hypulina Wehrli in its Host Larvae

XIE Gong-Yin ,
HU Cui

1.
Department of Plant Protection
2.
Zhejiang Agriculture University

Abstract

Effect of temperature on the growth dynamics of the nuclear polyhedrosis virus form Ectropis obliqua hypulina (EoNPV) in its host larvae was studied through polyhedra counting, counter immuno-electrophoresis, single immunodiffusion and rocket immuno-electrophoresis tests. The propagation of the virus in the host larvae fed with the NPV at the concentration of 2.6 10~7PIB/ml and reared at 26℃ was normal. The polyhedra titer or its relative equivalences increased positively with the time after inoculation and...
- Ectropis
- , obliqua hypulina
- , Nuclear polyhedrosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Nuclear Polyhedrosis Virus from Ectropis obliqua hypulina Wehrli in its Host Larvae

XIE Gong-Yin ,
HU Cui

1. Department of Plant Protection
2. Zhejiang Agriculture University

Abstract: Effect of temperature on the growth dynamics of the nuclear polyhedrosis virus form Ectropis obliqua hypulina (EoNPV) in its host larvae was studied through polyhedra counting, counter immuno-electrophoresis, single immunodiffusion and rocket immuno-electrophoresis tests. The propagation of the virus in the host larvae fed with the NPV at the concentration of 2.6 10~7PIB/ml and reared at 26℃ was normal. The polyhedra titer or its relative equivalences increased positively with the time after inoculation and...