HE Kong-Wang, LIN Ji-Huang, JIANG Jie-Yuan, Chen-Jiang-Ping, HUANG Bao-Shun, LI Bao-Tong and YANG Yu-Hua. Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus[J]. Virologica Sinica, 1990, 5(4).
Citation:
HE Kong-Wang, LIN Ji-Huang, JIANG Jie-Yuan, Chen-Jiang-Ping, HUANG Bao-Shun, LI Bao-Tong, YANG Yu-Hua.
Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus .VIROLOGICA SINICA, 1990, 5(4)
: 430.
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摘要
以纯化浓缩的HN-7株牛轮状病毒(牛RV,从河南腹泻犊黄牛粪样中分离)作为免疫原,免疫Balb/c小鼠,取其脾细胞与同系小鼠骨髓瘤细胞SP2/0融合,经克隆化筛选出2株(2C_7和3C_9)具群特异性的单克隆杂交瘤细胞株。2C_7与3C_9的小鼠腹水单克隆抗体(McAb)与牛HN-7、BRV007、BRV014及NCDV,猪Na86,猴SA-11和人Wa株RV细胞培养物的间接ELISA反应效价均达1∶10~5,但它们的HI和NT滴度分别≤1∶8和≤1∶100。McAb的Ig类别和IgC亚类检测结果2C_7为IgG1,3C_9为IgG2b。分别用3C_9 McAb和多克隆抗血清(PcAb)包被微量反应板进行粪样中RV抗原检测,共检测犊黄牛腹泻粪样36份、婴幼儿腹泻粪样40份,McAb和PcAb两系统检测结果的符合率分别达到97.2%(35/36)和100%(40/40)。
Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus
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1.
Institute of Animal Husbandry and Veterinary Medicine
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2.
Jiangsu Academy of Agricultural Sciences
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3.
Nanjing 210014Department of Microbiology
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Abstract
Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because the titers of two ascite McAbs were up to 1: 10~5 or above by indirect ELISA with RV of Bovine HN-7, BRV007, BRV014 and NCDV, Porcine Na86, Simian SA-11 and Human Wa strain, but were≤ 1: 8 and≤1: 100 respectively...
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References
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Proportional views
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