Comparison of Detected Results of Blood and Urine Specimens of Infant Patients with Hepatitis Using ~(32)p and Biotin-Labeled HCMV DNA Probes

CHAN Yi-Fei; JI Hong; QIU Mu-Rui; WANG De-Bao

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February 1991

CHAN Yi-Fei, JI Hong, QIU Mu-Rui and WANG De-Bao. Comparison of Detected Results of Blood and Urine Specimens of Infant Patients with Hepatitis Using ~(32)p and Biotin-Labeled HCMV DNA Probes[J]. Virologica Sinica, 1991, 6(1).

Citation: CHAN Yi-Fei, JI Hong, QIU Mu-Rui, WANG De-Bao. Comparison of Detected Results of Blood and Urine Specimens of Infant Patients with Hepatitis Using ~(32)p and Biotin-Labeled HCMV DNA Probes .VIROLOGICA SINICA, 1991, 6(1) : 39.

同位素与生物素标记的HCMV DNA探针检测婴儿肝炎综合症血和尿标本的比较

1.
上海医科大学生物物理教研室
2.
中国科学院上海生物化学研究所
3.
中国科学院上海生物化学研究所上海

摘要

用~(32)P和生物素标记的克隆化的人巨细胞病毒(HCMV)AD169株DNA片段作探针,采用DNA-DNA斑点杂交法,对照检测了27例婴儿肝炎综合症患者(血清学检测为非甲非乙型肝炎者)临床血、尿标本的HCMV-DNA。其中16份血标本呈阳性,占59％;9份尿标本呈阳性,占33％。初步结果表明,血标本中HCMV DNA检出率比尿标本检出率高26％。标记的~(32)P探针可检测10pg同源DNA,生物素探针可检测50Pg同源DNA,均不与其它疱疹病毒及未感染的人胚肺细胞DNA杂交。将其中26份血标本的HCMV DNA杂交结果与抗HCMV IgM ELISA检测结果相比较,符合率为65％。
- 人巨细胞病毒
- , （32）P标记探针
- , 生物素标记探针
- , DNA-DNA杂交
- , 抗HCMV IgM

Comparison of Detected Results of Blood and Urine Specimens of Infant Patients with Hepatitis Using ~(32)p and Biotin-Labeled HCMV DNA Probes

1.
Department of Biophysics
2.
Shanghai Medical University
3.
Shanghai 200032 ~*Shanghai Institute of Biochemistry

Abstract

Human cytomegaiovirus(HCMV)DNA in clinical blood and urine specimens of 27 infant patients infected with hepatitis were detected by DNA-DNA dot blot hybridization using ~(32)P and biotin labeled cloned HCMV AD169 DNA fragment. The hybridization results of blood and urine specimens were compared 16 of 27 blood specimens were pesitive(59％). Of 27 urine specimens, 9 were positive (33％). The preliminary results indicate that the positive rate of HCMV DNA in blood specimens is 26％ higher than that in urine speci...
- Southern blot technique Hepatitis B virus Liver White blood cell Type

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison of Detected Results of Blood and Urine Specimens of Infant Patients with Hepatitis Using ~(32)p and Biotin-Labeled HCMV DNA Probes

1. Department of Biophysics
2. Shanghai Medical University
3. Shanghai 200032 ~*Shanghai Institute of Biochemistry

Abstract: Human cytomegaiovirus(HCMV)DNA in clinical blood and urine specimens of 27 infant patients infected with hepatitis were detected by DNA-DNA dot blot hybridization using ~(32)P and biotin labeled cloned HCMV AD169 DNA fragment. The hybridization results of blood and urine specimens were compared 16 of 27 blood specimens were pesitive(59％). Of 27 urine specimens, 9 were positive (33％). The preliminary results indicate that the positive rate of HCMV DNA in blood specimens is 26％ higher than that in urine speci...