Studies on Some Biophysical and Biochemical Characteristics of Rabbit Hemorrhagic Disease Virus (RHDV) DHA

SUN Song-Bai; WANG Han-Zhong; YANG Hua-Lou; LUO Jing; LIU Hong

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February 1991

SUN Song-Bai, WANG Han-Zhong, YANG Hua-Lou, LUO Jing and LIU Hong. Studies on Some Biophysical and Biochemical Characteristics of Rabbit Hemorrhagic Disease Virus (RHDV) DHA[J]. Virologica Sinica, 1991, 6(1).

Citation: SUN Song-Bai, WANG Han-Zhong, YANG Hua-Lou, LUO Jing, LIU Hong. Studies on Some Biophysical and Biochemical Characteristics of Rabbit Hemorrhagic Disease Virus (RHDV) DHA .VIROLOGICA SINICA, 1991, 6(1) : 59.

兔出血症病毒核酸的某些理化性质的研究

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

本文对我国无锡分离的兔出血症病毒A_2R-3毒株核酸的某些理化性质进行了研究。采用孚尔根染色、二苯胺反应和核酸酶解实验证实病毒核酸为DNA类型。吖啶橙染色、甲醛反应、核酸酶S_1消化和核酸热变性实验表明病毒核酸为单链型。核酸电泳呈单一组分。电境观察显示核酸分子链呈线状,平均长度约为2.15μ。计算分子量约为2.1—2.5×10~6d。核酸碱基组盛为A25.34、T29.37、G23.85、C21.43、(G+C)克分子百分比值为45.28。结合以前的报道、我们认为:兔出血症病毒可以归类于细小病毒科。
- 兔出血症病毒
- , 单链DNA
- , 细小病毒

Studies on Some Biophysical and Biochemical Characteristics of Rabbit Hemorrhagic Disease Virus (RHDV) DHA

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

In present paper, some biophysical and biochemical characteristies of the nucleic acid in the original strain of the Rabbit Hemerrhagic Disease Virus (RHDV)-A_2R-3(separeted from Wuxi, China. 1984) have been studied. By using Feulgen assay, Diphenylamine reaction test and nucleate digestion sensitivity test, we demonstrated that the nucleic acid of the RHDV was DNA form. Staining with acridine orange, reaction with diluted formaldehyde, digestion with nuclease Sl and determination of the thermal denatur...
- Rabbit Hemorrhagic Disease Virus （RHDV） Parvovirus Single stranded DNA（ssDNA）

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Some Biophysical and Biochemical Characteristics of Rabbit Hemorrhagic Disease Virus (RHDV) DHA

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: In present paper, some biophysical and biochemical characteristies of the nucleic acid in the original strain of the Rabbit Hemerrhagic Disease Virus (RHDV)-A_2R-3(separeted from Wuxi, China. 1984) have been studied. By using Feulgen assay, Diphenylamine reaction test and nucleate digestion sensitivity test, we demonstrated that the nucleic acid of the RHDV was DNA form. Staining with acridine orange, reaction with diluted formaldehyde, digestion with nuclease Sl and determination of the thermal denatur...