Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ

LIU Ming-Fu; XIE Tian-En

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February 1991

LIU Ming-Fu and XIE Tian-En. Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ[J]. Virologica Sinica, 1991, 6(1).

Citation: LIU Ming-Fu, XIE Tian-En. Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ .VIROLOGICA SINICA, 1991, 6(1) : 75.

原位化学切割法对油桐尺蠖核型多角体病毒两种相似多肽的比较研究

刘明富 ,
谢天恩

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

油桐尺蠖核型多角体病毒(BsNPV)自1978年分离以来,对其形态结构、形态发生、毒力测定、血清学、理化特性、安全性试验、剂型研究及大田应用等已进行了广泛的研究,其基因组物理图谱也已构建。采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)研究BsNPV蛋白质时发现,多角体蛋白与病毒粒子的一种主要蛋白质的分子量非常接近
- 原位化学切割法
- , Asp-Pro肽键
- , 油桐尺蠖核型多角体病毒
- , 31K蛋白

Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ

LIU Ming-Fu ,
XIE Tian-En

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75％ formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the...
- In silu chemical cleavage Asp-Pro peptide bond Buzura suppressaria nuclear polyhedrosis virus （BsNPV） 31k protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparative Studies on Two Similar Proteins of Buzura suppressaria Nuclear Polyhedrosis Virus with Chemical Cleavage Method in situ

LIU Ming-Fu ,
XIE Tian-En

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75％ formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the...