ZHENG Ji-Shan, LI Fa-Qing, LIN Jian-Men, PAN Xiu-Zhen and TAN Wei-Guo. Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro[J]. Virologica Sinica, 1991, 6(1).
Citation: ZHENG Ji-Shan, LI Fa-Qing, LIN Jian-Men, PAN Xiu-Zhen, TAN Wei-Guo. Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro .VIROLOGICA SINICA, 1991, 6(1) : 79.

甲肝病毒在人血白细胞中体外连续传代和在分类白细胞中增殖的研究

  • 1985年我们采用间接免疫荧光法(IF法)检测出甲肝患者外周血白细胞中有甲肝抗原(HAAg)存在,继而又将HAAg阳性白细胞直接种入PLC/PRF/5细胞,分离到两株甲肝病毒(HAV)NJ—3株和H—1株。为了弄清白细胞所携带的病毒究竟仅为吸附吞饮,抑或能在其中复制增殖,我们将分离到的HAV用正常人血白细胞进行体外增殖试验,现将结果报告如下。

Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro

  • This paper reports the experimental studies on propagation of HAV in the human mononuclear leukocytes in vitro. The mononuelear leukocytes were separated from the peripheral blood of health person and the umbilical blood of fetus using Ficoll-Hypaque technique. The NJ-3 strain of HAV was inoclated into the adherent cells in culture flask and the suspensions of mononuclear leukocytes. After incubation the cultures were examined by indirect IF technique. It was found that both the adherent and nonadherent mon...

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    Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro

    • 1. Institute of Military Medicine
    • 2. Nanking Command

    Abstract: This paper reports the experimental studies on propagation of HAV in the human mononuclear leukocytes in vitro. The mononuelear leukocytes were separated from the peripheral blood of health person and the umbilical blood of fetus using Ficoll-Hypaque technique. The NJ-3 strain of HAV was inoclated into the adherent cells in culture flask and the suspensions of mononuclear leukocytes. After incubation the cultures were examined by indirect IF technique. It was found that both the adherent and nonadherent mon...

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