LIU Yan-Fang, YANG Shou-Jing, YAN Pei-Song and WANG Chun-Jie. Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells[J]. Virologica Sinica, 1991, 6(2).
Citation:
LIU Yan-Fang, YANG Shou-Jing, YAN Pei-Song, WANG Chun-Jie.
Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells .VIROLOGICA SINICA, 1991, 6(2)
: 113.
细胞中流行性出血热(EHF)病毒抗原部分弥散及其原位检测
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第四军医大学病理教研室
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第四军医大学病理教研室 西安
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摘要
用流行性出血热病人尸检及活检材料。以不同固定液、不同免疫细胞化学方法等对细胞中的EHFV抗原进行了检测。用目前我国自产的六种单克隆抗体(McAbs)单用或混用,只能定位出固定及时,近期保存标本中的抗原。在长期保存的尸检标本上及延期固定的活检标本上,光镜及电镜下均可见抗原有明确的弥散现象。用多克隆抗体(PcAb)配合高级敏感的免疫细胞化学方法可显示保存近三十年的陈旧尸检蜡块切片中残存的部分抗原。不同的固定剂、固定时间、免疫细胞化学方法、清除内源酶的时间及病期都对定位EHFV抗原有一定影响。
Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells
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Abstract
In the detecton of epidemic hemmorrhagic fever viral antigen in the autopsy and biopsy tissues by immunocytochemical methods, it was found that the monoclonal antibodies to nuclear antigen could stain antigen well with a rather diffuse pattern in fresh and well fixed material only, Polyclonal antibody could stain autopsy material imbedded in paraffin for even 28 years, as clear-cut vescular inclusion body. Probably the N antigen has been diffused away after long preservation and bad fixation. ABC, four fold...
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References
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Proportional views
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