Study of EHFV Directly Activating Complement C3 in Vitro

ZHOU Ning; NI Da-Shi; HE Hao; LI Yong-Zhen

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April 1991

ZHOU Ning, NI Da-Shi, HE Hao and LI Yong-Zhen. Study of EHFV Directly Activating Complement C3 in Vitro[J]. Virologica Sinica, 1991, 6(2).

Citation: ZHOU Ning, NI Da-Shi, HE Hao, LI Yong-Zhen. Study of EHFV Directly Activating Complement C3 in Vitro .VIROLOGICA SINICA, 1991, 6(2) : 121.

流行性出血热病毒体外直接激活补体C_3的研究

1.
安徽省医学科学研究所病毒研究室
2.
安徽省医学科学研究所病毒研究室合肥

摘要

用蔗糖梯度纯化的4株不同来源的流行性出血热(EHF)病毒(陈株、H8205、ALC96、R22)抗原,在体外直接与健康人血清于37℃作用30分钟,用交叉免疫电泳检测血清中C_3激活情况。结果陈株、H8205、R22 3株病毒抗原,可以直接激活补体C_3,电泳后呈现典型降解峰,而ALC96与正常细胞抗原则均为阴性。病毒抗原激活C_3的活性,与病毒核衣壳蛋白(Nucleocapsid Protein)的含量及毒株对乳鼠的半数致死量有关,并可被特异性抗体(IgG)所中和。
- 流行性出血热
- , 补体C_3
- , 体外激活

Study of EHFV Directly Activating Complement C3 in Vitro

1.
Anhui Institute of Medical Sciences
2.
Hefei 230061

Abstract

The antigens of four EHFV strains from different sources(Chen, H8205, ALC96, R22)purified by sucrose gradient centrifugation incubated with normal human sera in 37℃ for 30 minutes Cross-immunoelectrophoresis was used to detect whether complement C_3 was activated. The results showed that Chen, H8205, R22 strains antigens can directly activate C_3, typical degradative peak was found after electrophoresis. But ALC96 and normal antigen were negative. Ability of activating C_3 of EHFV antigens was related to th...
- Epidemic hemorrhagic fever
- , virus Complement C_3
- , Activation in vitro

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of EHFV Directly Activating Complement C3 in Vitro

1. Anhui Institute of Medical Sciences
2. Hefei 230061

Abstract: The antigens of four EHFV strains from different sources(Chen, H8205, ALC96, R22)purified by sucrose gradient centrifugation incubated with normal human sera in 37℃ for 30 minutes Cross-immunoelectrophoresis was used to detect whether complement C_3 was activated. The results showed that Chen, H8205, R22 strains antigens can directly activate C_3, typical degradative peak was found after electrophoresis. But ALC96 and normal antigen were negative. Ability of activating C_3 of EHFV antigens was related to th...