YOU Feng-Xin, LIU Bing-Hui, ZHANG Ling-Mei and DING Mao-Jin. Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method[J]. Virologica Sinica, 1991, 6(2).
Citation:
YOU Feng-Xin, LIU Bing-Hui, ZHANG Ling-Mei, DING Mao-Jin.
Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method .VIROLOGICA SINICA, 1991, 6(2)
: 125.
水标本中脊髓灰质炎病毒三种浓缩方法的比较及改进方法的建立
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摘要
本文用三种浓缩方法对人工污染标本进行了病毒回收率的比较试验,三种方法结果基本相同(47—49%)。在被检脊髓灰质炎Ⅰ型病毒蒸馏水标本中加1%NaCl可提高滤膜法对水中病毒的回收率;在含脊髓灰质炎病毒的河水、污水标本中加1%NaCl及AlCl_3(河水为0.0005mol/L、污水为0.001mol/L),离心沉淀过程中,我们惊奇地发现病毒全在沉渣中,结果表明用该方法病毒回收率竟提高了一倍(92—99%)。
Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method
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Abstract
This paper described the quantative eomprison of 3 methods concentrating poliovirus type 1 artificially added into the water samples, The preliminary results showed that the recovery rate of the virus in the water samples was similar (47—49%)among the 3 methods, It was discovered that the addition of 1% NaCl into virus contaminted distilled water sample increased significantly the virus-recovery rate with milliporous membrane filtrating method and we established an improved process of virus concentration of...
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