Propagation of EHFV in the Wish Cell Line

TAN Wei-Guo; LI De-Qing; ZHOU Li-Gong

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Volume 06 Issue 2

April 1991

TAN Wei-Guo, LI De-Qing and ZHOU Li-Gong. Propagation of EHFV in the Wish Cell Line[J]. Virologica Sinica, 1991, 6(2).

Citation: TAN Wei-Guo, LI De-Qing, ZHOU Li-Gong. Propagation of EHFV in the Wish Cell Line .VIROLOGICA SINICA, 1991, 6(2) : 179.

流行性出血热病毒在人革膜传代细胞内的生长繁殖

1.
南京军区军事医学院研究所
2.
南京军区军事医学院研究所南京

摘要

本文应用人羊膜传代细胞(Wish细胞)对流行性出血热病毒(EHFV)的敏感性进行了试验,用EHFV武株及76118、A9、R22、R4、陈株等5个代表株接种Wish细胞。结果Wish细胞感染EHFV后第1代第8～10天即可查见明显的特异性荧光,荧光光强度随代数和时间而增加,TCID50为10~4～/10~5ml。并用荧光阻断试验和双份血清证实在Wish细胞内繁殖的病毒为EHFV。此结果为EHF病原学研究提供了新的敏感细胞株。
- 流行性出血热病毒
- , 人羊膜传代细胞

Propagation of EHFV in the Wish Cell Line

1.
Institute of Military Medicine
2.
Nanjing Command

Abstract

In our experimental studies, 6 strains of EHFV were inoculated into the Wish cell cultures. We found that EHFV could propagate in this cell line and after several passages, viral titers can reach to 10~(4-5) TCID50/ml. After incubation no cell cytopathic effect could be observed in the Wish cell cultures. The result showed that the Wish cell is a new sensitive cell line for isolation of EHFV.
- wish cell line EHFV
- , Human amnion continues cell line

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Propagation of EHFV in the Wish Cell Line

1. Institute of Military Medicine
2. Nanjing Command

Abstract: In our experimental studies, 6 strains of EHFV were inoculated into the Wish cell cultures. We found that EHFV could propagate in this cell line and after several passages, viral titers can reach to 10~(4-5) TCID50/ml. After incubation no cell cytopathic effect could be observed in the Wish cell cultures. The result showed that the Wish cell is a new sensitive cell line for isolation of EHFV.