Observation of HFRSV Inclusion Bodies under Ordinary Optical Microscope with APAAP Immunohistochemical Technique in Two HFRSV Sensitive Cell Lines

HUANG Chuan-Shu; JIN Bo-Quan; WANG Mei-Xian; LIU Jian; ZHAO Ning

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June 1991

HUANG Chuan-Shu, JIN Bo-Quan, WANG Mei-Xian, LIU Jian and ZHAO Ning. Observation of HFRSV Inclusion Bodies under Ordinary Optical Microscope with APAAP Immunohistochemical Technique in Two HFRSV Sensitive Cell Lines[J]. Virologica Sinica, 1991, 6(3).

Citation: HUANG Chuan-Shu, JIN Bo-Quan, WANG Mei-Xian, LIU Jian, ZHAO Ning. Observation of HFRSV Inclusion Bodies under Ordinary Optical Microscope with APAAP Immunohistochemical Technique in Two HFRSV Sensitive Cell Lines .VIROLOGICA SINICA, 1991, 6(3) : 201.

应用APAAP光镜技术发现两株HFRSV敏感细胞中HFRSV包涵体

1.
第四军医大学免疫学教研室
2.
第四军医大学中心实验室
3.
第四军医大学免疫学教研室西安

摘要

本文应用抗HFRSV McAb的APAAP(碱性磷酸酶—抗碱性磷酸酶)免疫组化技术,首次用光镜在HFRSV感染8—14天的Hep-2和wish细胞中查出病毒包涵体,并且根据其位置和形态特征可分为:(1)中央型包涵体;(2)外周型包涵体;(3)脱落包涵体。上述所见包涵体均在电镜下得到证实。此结果对于了解HFRSV感染细胞后的致病变作用以及研究HFRSV感染细胞包涵体的特征、来源、性质和形成过程具有十分重要的意义。
- HFRSV
- , 包涵体
- , APAAP

Observation of HFRSV Inclusion Bodies under Ordinary Optical Microscope with APAAP Immunohistochemical Technique in Two HFRSV Sensitive Cell Lines

1.
Department of Immunology
2.
The Fourth Military Medical University
3.
Xi-an

Abstract

Investigation of the HFRSV inclusion bodies was carried out under ordinary optical microscope with APAAP(alkaline phosphatase anti-alkaline phosphatase)immunohistochemical technique in the Hep-2 and Wish cell lines infected by HFRSV (Hemorrhagic fever with Renal syndrome virus). It was found that there are three types of virus inclusion bodies, central, pheripheral and shedding types. All these findings were verified by electron microscopy, These results have great significance for further understanding the...
- HFRSV
- , Inclusion
- , APAAP

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Observation of HFRSV Inclusion Bodies under Ordinary Optical Microscope with APAAP Immunohistochemical Technique in Two HFRSV Sensitive Cell Lines

1. Department of Immunology
2. The Fourth Military Medical University
3. Xi-an

Abstract: Investigation of the HFRSV inclusion bodies was carried out under ordinary optical microscope with APAAP(alkaline phosphatase anti-alkaline phosphatase)immunohistochemical technique in the Hep-2 and Wish cell lines infected by HFRSV (Hemorrhagic fever with Renal syndrome virus). It was found that there are three types of virus inclusion bodies, central, pheripheral and shedding types. All these findings were verified by electron microscopy, These results have great significance for further understanding the...