MI Ke-Yong, WANG Qiang, ZHANG Fang-Dong and XIANG Xiu-Cheng. cDNA Synthesis of BVDV RNA and the Research of Its Hybridization[J]. Virologica Sinica, 1991, 6(3).
Citation: MI Ke-Yong, WANG Qiang, ZHANG Fang-Dong, XIANG Xiu-Cheng. cDNA Synthesis of BVDV RNA and the Research of Its Hybridization .VIROLOGICA SINICA, 1991, 6(3) : 242.

牛病毒性腹泻病毒RNA的cDNA合成及其分子杂交的研究

cDNA Synthesis of BVDV RNA and the Research of Its Hybridization

  • The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and agarose gel eleetrophoresis The purified BVDV RNA was polyadenylated at the 3′end under the catalysis of poly A polymerase, eDNA was synthesized with reverse transcriptase using the poly A-tailed BVDV RNA as template...

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    cDNA Synthesis of BVDV RNA and the Research of Its Hybridization

    • 1. Wuhan Institute of Virology
    • 2. Academia Sinica

    Abstract: The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and agarose gel eleetrophoresis The purified BVDV RNA was polyadenylated at the 3′end under the catalysis of poly A polymerase, eDNA was synthesized with reverse transcriptase using the poly A-tailed BVDV RNA as template...

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