Identification of the Morphological Transforming Regions of Human Cytomegalovirus

Leonard J Rosenthal; Shang Shi-zhang; Anita Inamdar

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August 1991

Leonard J Rosenthal, Shang Shi-zhang and Anita Inamdar. Identification of the Morphological Transforming Regions of Human Cytomegalovirus[J]. Virologica Sinica, 1991, 6(4).

Citation: Leonard J Rosenthal, Shang Shi-zhang, Anita Inamdar. Identification of the Morphological Transforming Regions of Human Cytomegalovirus .VIROLOGICA SINICA, 1991, 6(4) : 281.

人巨细胞病毒形态转化区的鉴定

1.
Department of Microbiology Georgetown University Medical Center Washington
2.
Jinan University Medical College
3.
Department of Microbiology Georgetown University Medical Center Washington
4.
D.C.20007 USA

摘要

DNA of human cytomegalovirus (HCMV) contains three transforming fragments, which have been mapped in the long unique region of the viral genome(see Fig.1). A minimal region of 558 base pairs(bp) (pCM4127) was localized in the XbaI-Hindlll fragment of HCMV strain AD169 (mapunit 0.123—0.140)and designated morphological transforming region Ⅰ (mtrⅠ). MtrⅠwas reported to cause one-step focal transformation of primary Wistar
- 人巨细胞病毒
- , 转化活性
- , DNA序列分析
- , Southern印迹
- , S_1核酸酶分析
- , 启动子活性
- , 氯霉素乙烯转移酶测定
- , NIH 3T3细胞

Identification of the Morphological Transforming Regions of Human Cytomegalovirus

1.
Department of Microbiology Georgetown University Medical Center Washington
2.
Jinan University Medical College
3.
Department of Microbiology Georgetown University Medical Center Washington
4.
D.C.20007 USA

Abstract

DNA of human cytomegalovirus (HCMV) contains three transforming fragments, which have been mapped in the long unique region of the viral genome(see Fig.1). A minimal region of 558 base pairs(bp) (pCM4127) was localized in the XbaI-Hindlll fragment of HCMV strain AD169 (mapunit 0.123—0.140)and designated morphological transforming region Ⅰ (mtrⅠ). MtrⅠwas reported to cause one-step focal transformation of primary Wistar
- Human Cytomegaloviruses Transformation Activity DNA Sequenee Analysis Southern Blot Hybridization S_1 Nuclease Analysis NIH 3T3 Cells Promoter Activity Chloramphenicol Acetyltransferase Assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification of the Morphological Transforming Regions of Human Cytomegalovirus

1. Department of Microbiology Georgetown University Medical Center Washington
2. Jinan University Medical College
3. Department of Microbiology Georgetown University Medical Center Washington
4. D.C.20007 USA

Abstract: DNA of human cytomegalovirus (HCMV) contains three transforming fragments, which have been mapped in the long unique region of the viral genome(see Fig.1). A minimal region of 558 base pairs(bp) (pCM4127) was localized in the XbaI-Hindlll fragment of HCMV strain AD169 (mapunit 0.123—0.140)and designated morphological transforming region Ⅰ (mtrⅠ). MtrⅠwas reported to cause one-step focal transformation of primary Wistar