SONG Hai-Feng, GUO Li-Li, SUN Zhong-He and ZHU Guan-Fu. Detection of HIV-1 DNA by PCR[J]. Virologica Sinica, 1991, 6(4).
Citation: SONG Hai-Feng, GUO Li-Li, SUN Zhong-He, ZHU Guan-Fu. Detection of HIV-1 DNA by PCR .VIROLOGICA SINICA, 1991, 6(4) : 324.

利用PCR法检测HIV—1 DNA

  • 通过比较多个HIV(人免疫缺陷病毒)分离株的核苷酸序列,我们选择膜基因上7373—7514位的一段保守区为目的片段合成了引物1(5′—AGCAGCAGGAAGCACTATGGGC—3′)和引物2(5′—CCAGACTGTGAGTTGCAACA—3′),并分别以质粒PⅢexE7和MT4-HIV-1 DNA,为模板进行了PCR反应及敏感性试验。结果表明,用PCR法可检测出1~10个质粒分子及1×10~6个细胞中一个感染细胞。因此我们推论,本法可应用于AIDS临床标本的检测。

Detection of HIV-1 DNA by PCR

  • A single pair of oligonucleotide primers within the envelope gene of HIV1(primer 1: 5 -AGCAGCAGGAAGCACTATGGGC-3 ; primer 2: 5 -CCAGACTGAGTTGCAACA-3 ), 142bp apart, were synthesized and used to amplify a specific fragment of the HIV-1 DNA in infected cell cultures(MT4 HIV 1), and in plasmid pⅢexE7 carrying HIV-1 envelope gene by means of polymerase chain reaction(PCR). Amplified product was identified as 142bp fragment by agrose electrophoresis and cut into a 50bp and a 92bp fragment with restriction endonuc...

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    Detection of HIV-1 DNA by PCR

    • 1. Institute of Microbiology and Epidemiology
    • 2. Beijing

    Abstract: A single pair of oligonucleotide primers within the envelope gene of HIV1(primer 1: 5 -AGCAGCAGGAAGCACTATGGGC-3 ; primer 2: 5 -CCAGACTGAGTTGCAACA-3 ), 142bp apart, were synthesized and used to amplify a specific fragment of the HIV-1 DNA in infected cell cultures(MT4 HIV 1), and in plasmid pⅢexE7 carrying HIV-1 envelope gene by means of polymerase chain reaction(PCR). Amplified product was identified as 142bp fragment by agrose electrophoresis and cut into a 50bp and a 92bp fragment with restriction endonuc...

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