Analysis of the Variations of Genomic RNA Patterns of Rotavirus

ZHANG Jian-Qiong; MENG Ji-Hong; LIN Ling

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August 1991

ZHANG Jian-Qiong, MENG Ji-Hong and LIN Ling. Analysis of the Variations of Genomic RNA Patterns of Rotavirus[J]. Virologica Sinica, 1991, 6(4).

Citation: ZHANG Jian-Qiong, MENG Ji-Hong, LIN Ling. Analysis of the Variations of Genomic RNA Patterns of Rotavirus .VIROLOGICA SINICA, 1991, 6(4) : 329.

轮状病毒RNA基因图谱变异分析

1.
南京铁道医学院微生物学教研室
2.
南京铁道医学院微生物学教研室南京

摘要

用聚丙烯酰胺凝胶电泳法对轮状病毒(RV)标准株进行RNA基因图谱分析,发现人轮状病毒(HRV)Wa株在MA104细胞上连续传5代后,其RNA基因图谱由原来的4:2:3:2模式变为4:3:3:2模式。继续分别在CV-1、MA104细胞上传代后,基因图谱进一步演变为4:3:4:2模式。经比较电泳、病毒空斑纯化,初步证实RVWa有变异林存在。目前尚未见RV标准株变异的报告,有待进一步研究。同时对多个RV标准株及1939年南京市区婴幼儿秋季腹泻的RV流行株进行基因图谱分析,发现不同的人RV及牛RV标准株有相同的基因图谱;不同实验室来源的同一标准株有不同的基因图谱。此外,尚证实HRV是1989年南京市区婴幼儿秋季腹泻的主要病源,总检出率45.1％,电泳长型为流行优势株89.2％。本文尚对RV RNA基因图谱变异的原因及意义进行了讨论。
- 轮状病毒
- , 聚丙烯酰胺凝胶电泳
- , 基因图谱分析
- , 变异

Analysis of the Variations of Genomic RNA Patterns of Rotavirus

1.
Department of Microbiology
2.
Nanjing Railway Medical College

Abstract

Using PAGE method, we analysed genomic RNA patterns of several standard strains of rotavirus including Wa, Ito, Yo Hochi, ST, SA-11 and UK strains, and the epidemic strains of rotavirus which caused infant diarrhoea in Nanjing in October to December 1989. It was interesting that the electrophoretic model of Wa strain had changed from 4:2:3:2 to 4:3:3:2, and then to 4:3:4:2 after it was cultured on MA104 or CV-1 cells for several passages. Three variants were cloned by the plaque assay from the "Wa" strain w...
- Rotavirus Variation
- , PAGE Analysis of Genomic Patterns

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of the Variations of Genomic RNA Patterns of Rotavirus

1. Department of Microbiology
2. Nanjing Railway Medical College

Abstract: Using PAGE method, we analysed genomic RNA patterns of several standard strains of rotavirus including Wa, Ito, Yo Hochi, ST, SA-11 and UK strains, and the epidemic strains of rotavirus which caused infant diarrhoea in Nanjing in October to December 1989. It was interesting that the electrophoretic model of Wa strain had changed from 4:2:3:2 to 4:3:3:2, and then to 4:3:4:2 after it was cultured on MA104 or CV-1 cells for several passages. Three variants were cloned by the plaque assay from the "Wa" strain w...