Studies on the Antisera to Narcissus Viruses

XIE Lian-Hui; ZHENG Xiang-Xiang; LIN Qi-Ying

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August 1991

XIE Lian-Hui, ZHENG Xiang-Xiang and LIN Qi-Ying. Studies on the Antisera to Narcissus Viruses[J]. Virologica Sinica, 1991, 6(4).

Citation: XIE Lian-Hui, ZHENG Xiang-Xiang, LIN Qi-Ying. Studies on the Antisera to Narcissus Viruses .VIROLOGICA SINICA, 1991, 6(4) : 344.

水仙病毒血清学研究 Ⅰ.水仙花叶病毒抗血清的制备及其应用

1.
福建农学院病毒室
2.
福建农学院病毒室福州

摘要

经人工接种分离纯化的水仙花叶病毒(NMV),以表现局斑的苋色藜叶片为提纯材料,经聚乙二醇(PEG_(6000))沉淀、差速离心和蔗糖密度梯度离心提纯后,进行6次家兔免疫注射制备抗血清。结果表明注射后3周左右效价最高,经毛细管沉淀方法测定可达1:5,000。用琼脂双扩散方法将此NMV抗血清与来自荷兰的NMV抗血清进行比较,结果发现二者的抗体性相同。应用此法制成的NMV抗血清,以琼脂双扩散、ELISA方法分别对30份水仙组培脱毒试管苗样本进行带毒率检测,结果表明所测得的平均带毒率分别为33.3％和80.0％,并用IEM测定方法验证了ELISA结果的可靠性和准确性。
- 水仙花叶病毒
- , 抗血清
- , 制备与应用

Studies on the Antisera to Narcissus Viruses

1.
Lab. of Plant Virology
2.
Fujian Agricultural College

Abstract

Narcissus mosaic virus(NMV) was obtained from the mixed infected narcissus plants and purified from the inoculated leaves of Chenopodium amarnticolor. The procedures of purification involved precipitation with 6％ PEG ( M. W. 6000 ), differential centrifugation and 20—50% sucrose density-gradient centrifugation. After 6 times of immune injection to rabbit with the purified preparation of NMV, an antiserum was prepared with a titre of 1:5000 tested by precipitation reactions in capillary pipettes about 4 week...
- Narcissus Mosaic Virus
- , PreParation and use of antiserum

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on the Antisera to Narcissus Viruses

1. Lab. of Plant Virology
2. Fujian Agricultural College

Abstract: Narcissus mosaic virus(NMV) was obtained from the mixed infected narcissus plants and purified from the inoculated leaves of Chenopodium amarnticolor. The procedures of purification involved precipitation with 6％ PEG ( M. W. 6000 ), differential centrifugation and 20—50% sucrose density-gradient centrifugation. After 6 times of immune injection to rabbit with the purified preparation of NMV, an antiserum was prepared with a titre of 1:5000 tested by precipitation reactions in capillary pipettes about 4 week...