Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA

CHEN Jian-Ping; RUAN Yi-Li

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August 1991

CHEN Jian-Ping and RUAN Yi-Li. Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA[J]. Virologica Sinica, 1991, 6(4).

Citation: CHEN Jian-Ping, RUAN Yi-Li. Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA .VIROLOGICA SINICA, 1991, 6(4) : 356.

应用F(ab )_2~-酶联吸附分析法检测大麦黄花叶病毒

陈剑平 ,
阮义理

1.
浙江省农业科学院病毒学实验室
2.
浙江省农业科学院病毒学实验室杭州

摘要

F(ab′)_2酶联免疫吸附分析法(F(ab′)_2-ELISA)成功地用于大麦黄花叶病毒(BaYMV)的常规检测和诊断.其步骤是先用稀释1000—4000倍的抗血清F(ab′)_2包被反应板,加待测样品和稀释1000倍的同种抗血清或IgG,然后再加A蛋白碱性磷酸酯酶和底物,测定OD值。比较试验表明,ELISA稀释缓冲液加入1％小牛血清或1％全脂奶粉,BaYMV的测检灵敏度可提高达2.5—5.0ng/ml,病叶汁液检测终浓度为稀释1600—3200倍。我国BaYMV分离物与英国分离物的血清学性质完全一致。BaYMV在大麦病株中以叶部含量较高,茎中含量次之,根部测不出病毒。检测和诊断田间样品,即使有的样品已不新鲜,也均能得到满意的结果。此方法也成功地用于大麦温和花叶病毒(BaMMV)、小麦黄花叶病毒(WYMV)、燕麦花叶病毒(OMV)和燕麦金色条纹病毒(OGSV)等禾谷多粘菌传麦毒的检测,这S种病毒的血清学关系研究表明,除BaYMV和WYMV之间具有血清学关系以外,其余彼此均不反应。
- F（ab′）_2酶联免疫吸附分析法
- , 大麦黄花叶病毒
- , 菌传麦类病毒
- , 血清学关系

Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA

1.
Virology Laboratory
2.
Zhejiang Academy of Agricultural Sciences

Abstract

Barley yellow mosaic virus(BaYMV) was routinely detected and diagnosed by the F(ab )_2 indirect enzyme-linked immunosorbent assaya F(ab )_2-ELISA in which (1) virus in ELISA buffer was trapped by F(ab )_2 fragments of speeifie IgG diluted in 1000 to 4000 with 0.05mol/L sodium carbonate coating buffer pH9.6 on microtiter plate, (2)trapped virus was detected by intact IgG diluted 1in 1000 with ELISA buffer, (3) positive reaction was identified using protein diluted A alkalinephosphatase diluted 1 in 1000 to 2...
- mosaic virus Fungally transmitted cereal viruses Serological relationship

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA

1. Virology Laboratory
2. Zhejiang Academy of Agricultural Sciences

Abstract: Barley yellow mosaic virus(BaYMV) was routinely detected and diagnosed by the F(ab )_2 indirect enzyme-linked immunosorbent assaya F(ab )_2-ELISA in which (1) virus in ELISA buffer was trapped by F(ab )_2 fragments of speeifie IgG diluted in 1000 to 4000 with 0.05mol/L sodium carbonate coating buffer pH9.6 on microtiter plate, (2)trapped virus was detected by intact IgG diluted 1in 1000 with ELISA buffer, (3) positive reaction was identified using protein diluted A alkalinephosphatase diluted 1 in 1000 to 2...