Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus

WU Yuan-Hua; WUI Shi-Quan

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August 1991

WU Yuan-Hua and WUI Shi-Quan. Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus[J]. Virologica Sinica, 1991, 6(4).

Citation: WU Yuan-Hua, WUI Shi-Quan. Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus .VIROLOGICA SINICA, 1991, 6(4) : 365.

草莓伪温和黄边病毒提纯技术的研究

吴元华 ,
韦石泉

1.
沈阳农业大学
2.
沈阳农业大学沈阳

摘要

纯化的草莓伪温和黄边病毒(SPMYEV),小叶嫁接繁殖于指示植物草莓UC—4上,然后采样榨汁,结合聚乙二醇(PEG)沉淀,蔗糖垫底差速离心及连续性蔗糖密度梯度离心,能成功地将草莓伪温和黄边病毒提纯出来。经电镜观察为丝杆状病毒粒体,长×宽为650nm×12—13nm。提纯的病毒制剂,经紫外分光光度计测定,呈典型的核蛋白吸收曲线,其最大值位于263nm,最小值位于243nm。A260/A280=1.24,用冰醋酸分解病毒,提取衣壳蛋白,经SDS-聚丙烯酰胺凝胶电泳测定其分子量约37000道尔顿。
- 草莓伪温和黄边病毒
- , 提纯程序
- , 衣壳蛋白

Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus

1.
Shenyang Agricultural University
2.
Shenyang 110161

Abstract

The isolated strawberry pseudo-mild yellow edge virus(SPMYEV)was maintained in the strawberry-Fragaria vesca L.var. UC-4, and was successfully extracted and purified through homogenization by polyethyleneglycol (PEG) precipitation, differential centrifugation in the sucrose cushion and continuous suerose density gradient centrifugation. The purified preparations cotained flexous rod-shaped virus particles mainly 650nm in length and 12—13nm in width. The virus showed a typitical UV spectrum of nueleoprotein ...
- Strawberry Pseudo-Mild Yellow Edge Virus SPMYEV Protedfure of plant virus purification Coat protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus

1. Shenyang Agricultural University
2. Shenyang 110161

Abstract: The isolated strawberry pseudo-mild yellow edge virus(SPMYEV)was maintained in the strawberry-Fragaria vesca L.var. UC-4, and was successfully extracted and purified through homogenization by polyethyleneglycol (PEG) precipitation, differential centrifugation in the sucrose cushion and continuous suerose density gradient centrifugation. The purified preparations cotained flexous rod-shaped virus particles mainly 650nm in length and 12—13nm in width. The virus showed a typitical UV spectrum of nueleoprotein ...