Studies on Fine Structure and Some Physico--chemical Characteristics of a Cytoplasmic Polyhedrosis Virus from Dendrolimus punctatus Wenshanensis

LIU Run-Zhong; XIE Tian-En; PENG Hui-Yin; CHEN Xin-Wen; CHEN Shi-Wei

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February 1992

LIU Run-Zhong, XIE Tian-En, PENG Hui-Yin, CHEN Xin-Wen and CHEN Shi-Wei. Studies on Fine Structure and Some Physico--chemical Characteristics of a Cytoplasmic Polyhedrosis Virus from Dendrolimus punctatus Wenshanensis[J]. Virologica Sinica, 1992, 7(1).

Citation: LIU Run-Zhong, XIE Tian-En, PENG Hui-Yin, CHEN Xin-Wen, CHEN Shi-Wei. Studies on Fine Structure and Some Physico--chemical Characteristics of a Cytoplasmic Polyhedrosis Virus from Dendrolimus punctatus Wenshanensis .VIROLOGICA SINICA, 1992, 7(1) : 69.

文山松毛虫质型多角体病毒形态结构及理化性质的研究

1.
中国科学院武汉病毒研究所
2.
云南省林科院武汉

摘要

对文山松毛虫质型多角体病毒的形态结构及理化特性进行了研究,多角体大部分为六边形,少数为四边形及近园形,其大小在0.47～2.45μ之间,平均为1.1μ。病毒粒子呈球形,无囊膜,致密的核芯区由一层外壳包裹,直径为60nm。病毒粒子表面有12个刺突,放大图象可见其亚单位排列。多角体蛋白的主要成分为一种,分子量为26200道尔顿,多角体蛋白氨基酸组成中不含半胱氨酸;其碱性氨基酸与酸性氨基酸之比为1:2.16。病毒粒子结构蛋白含五条多肽组分。用SDS-热酚法提取所得核酸,其热变性紫外吸收OD_(260)值增加51.6％。抗核酸酶S_1。Tm值为86℃。在1％琼脂糖凝胶电泳中可分为9个片段,而在5％PAGE中,则可分为10个片段。各片段大小在0.66×10~6～2.85×10~6道尔顿之间,总分子量为15.35×10~6。电镜分析研究显示了CPV RNA在0.4μ、0.8μ和1.2μ处有三个分布峰。
- 文山松毛虫
- , 质型多角体病毒
- , 形态结构
- , 理化特性

Studies on Fine Structure and Some Physico--chemical Characteristics of a Cytoplasmic Polyhedrosis Virus from Dendrolimus punctatus Wenshanensis

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant differenceranging from 0.47 to 2.45μ and 1.1μ in average in diameter. Virions werespherical in shape with six spikes Iocated and icosahedral vertices, measuring60nm in diameter. Magnifying the photograph, the subunit arrangement...
- 文山松毛虫
- , 质型多角体病毒
- , 形态结构
- , 理化特性

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Fine Structure and Some Physico--chemical Characteristics of a Cytoplasmic Polyhedrosis Virus from Dendrolimus punctatus Wenshanensis

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant differenceranging from 0.47 to 2.45μ and 1.1μ in average in diameter. Virions werespherical in shape with six spikes Iocated and icosahedral vertices, measuring60nm in diameter. Magnifying the photograph, the subunit arrangement...