Purification and Partial characterization of Maize Dwarf Mosaic Virus

CHEN Gang; ZHOU Guang-He; CHEN Jian-Bo

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February 1992

CHEN Gang, ZHOU Guang-He and CHEN Jian-Bo. Purification and Partial characterization of Maize Dwarf Mosaic Virus[J]. Virologica Sinica, 1992, 7(1).

Citation: CHEN Gang, ZHOU Guang-He, CHEN Jian-Bo. Purification and Partial characterization of Maize Dwarf Mosaic Virus .VIROLOGICA SINICA, 1992, 7(1) : 93.

玉米矮花叶病毒提纯及特性的研究

1.
中国农业科学院植物保护研究所
2.
中国农业科学院植物保护研究所北京

摘要

改进病毒纯化方法,获得了较高纯度、较强侵染活性的提纯病毒制品。提纯病毒的产量为0.71.2mg/100g病叶,病毒粒体大小为72075014nm,SDS-聚丙烯酰胺凝胶电泳测得的外壳蛋白分子量为36000道尔顿,用提纯病毒免疫家兔得到较高特异性的抗血清,微量沉淀反应的效价为1/2048,间接ELISA法测出的感病叶汁液的最高稀释倍数为32006400倍。
- 小苍兰褪绿条纹花叶病毒
- , 病毒特性

Purification and Partial characterization of Maize Dwarf Mosaic Virus

1.
Institute of plant protection
2.
CAAS

Abstract

Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...
- MDMV
- , Purification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Purification and Partial characterization of Maize Dwarf Mosaic Virus

1. Institute of plant protection
2. CAAS

Abstract: Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...