Preliminary Report of Cell Culture of Hepatitis E Virus

YIN Shu-Rong; LIU Yu-Tang; TIAN Xin; BAO Zuo-Yi; JIANG Yu-Tu; ZHANG Xi-Tan

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April 1992

YIN Shu-Rong, LIU Yu-Tang, TIAN Xin, BAO Zuo-Yi, JIANG Yu-Tu and ZHANG Xi-Tan. Preliminary Report of Cell Culture of Hepatitis E Virus[J]. Virologica Sinica, 1992, 7(2).

Citation: YIN Shu-Rong, LIU Yu-Tang, TIAN Xin, BAO Zuo-Yi, JIANG Yu-Tu, ZHANG Xi-Tan. Preliminary Report of Cell Culture of Hepatitis E Virus .VIROLOGICA SINICA, 1992, 7(2) : 134.

细胞分离戊型肝炎病毒的初步研究

1.
军事医学科学院微生物流行病研究所
2.
军事医学科学院微生物流行病研究所北京

摘要

本文报告用2BS细胞分离戊型肝炎病毒(HEV)感染成功的猴肝脏标本中的HEV的初步结果,将2BS细胞长成致密单层,接种已知含有HEV的肝悬液,盲传至第4代,发现在第27天细胞出现了轻微的细胞病变(CPE),随着进一步的传代,细胞病变时间逐渐提前。至第7代时,在第17天就可见到CPE,CPE的主要特征是细胞间隙变宽,透明度降低和轻度细胞破坏,无大片细胞溶解和脱落现象。免疫电镜检查在第5代和第6代细胞培养液中见到很少的27—34nm的病毒颗粒。将第4代至第7代的细胞悬液20ml接种了3只恒河猴,有2只猴在24天和26天出现了典型的ALT升高,待这3只猴恢复正常后一年,再次接种以上细胞悬液20ml,3只猴全部发生了ALT升高,并在胆汁中检查到27—34nm的病毒颗粒,我们初步推测2BS适应的这株病毒很可能是戊型肝炎病毒。
- 戊型肝炎病毒
- , 细胞培养
- , 2BS细胞

Preliminary Report of Cell Culture of Hepatitis E Virus

1.
Institute of Microbiology and Epdemiology
2.
Academy of Military Medical Sciences

Abstract

Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of 27—34nm virus-like particles(VLPs) in cell cultures were visualized by immunoelectronmicroseopy(IEM). 2 of 3 rhesus monkeys inoculated with 20ml of pool of cell cultures from 4 to 7 passages developed significant eleva...
- Hepatitis E virus Cell culture Human embryomic lung cell

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preliminary Report of Cell Culture of Hepatitis E Virus

1. Institute of Microbiology and Epdemiology
2. Academy of Military Medical Sciences

Abstract: Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of 27—34nm virus-like particles(VLPs) in cell cultures were visualized by immunoelectronmicroseopy(IEM). 2 of 3 rhesus monkeys inoculated with 20ml of pool of cell cultures from 4 to 7 passages developed significant eleva...