A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application

JIANG Ke-Qiang; PENG Qin-Lin; E Hai-Chun

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April 1992

JIANG Ke-Qiang, PENG Qin-Lin and E Hai-Chun. A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application[J]. Virologica Sinica, 1992, 7(2).

Citation: JIANG Ke-Qiang, PENG Qin-Lin, E Hai-Chun. A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application .VIROLOGICA SINICA, 1992, 7(2) : 138.

ABC-ELISA检测抗HBc的方法及其在临床和流行病学中的初步应用

1.
海军医学研究所
2.
海军医学研究所上海

摘要

应用生物素与抗生物素系统酶联免疫吸附试验建立了检测乙型病毒性肝炎患者血清中抗HBc的ABC-ELISA方法并与普通ELISA法进行了比较。结果表明:本法敏感性较普通ELISA法高4倍,阳性检出率提高了42.42％,且具有较好的重复性。将乙肝不同抗原、抗体进行替代试验和用纯化抗HBc-lgG进行抑制试验,证明本法有较高的特异性。将本法制备成试剂盒,并与上海市传染病院,静华公司及科华公司生产的普通ELISA试剂盒进行了比较,结果本试剂盒阳性检出率分别提高了25.00％,45.28％和30.77％。经上海市三个医院临床标本试验表明,本法具有快速、敏感、特异及稳定等优点。从而为抗HBc的检测提供了一种敏感的方法。
- 生物素抗生物素系统
- , 酶联免疫吸附试验（ABC-ELISA）
- , 抗HBc

A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application

1.
Naval Medical Research Institute
2.
Shanghai 200433

Abstract

The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means of ABC-ELISA increased by 42.42% over the one by using conventional ELISA. Repeated experiments showed good reproductivity. Alternative tests using various antigens and antibodies of HBV and blockage test using p...
- Biotin-Avidin System ABC-ELISA Anti-HBc

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application

1. Naval Medical Research Institute
2. Shanghai 200433

Abstract: The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means of ABC-ELISA increased by 42.42% over the one by using conventional ELISA. Repeated experiments showed good reproductivity. Alternative tests using various antigens and antibodies of HBV and blockage test using p...