Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test

YU Yong-Xin; YAO Zhi-Hui; AN Qi

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April 1992

YU Yong-Xin, YAO Zhi-Hui and AN Qi. Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test[J]. Virologica Sinica, 1992, 7(2).

Citation: YU Yong-Xin, YAO Zhi-Hui, AN Qi. Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test .VIROLOGICA SINICA, 1992, 7(2) : 156.

出血热病毒株的分型和抗原性比较的进一步研究

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京

摘要

本工作进一步对分离自不同来源的14株出血热病毒用空斑减少中和试验(PRNT)方法进行了分型。以不同株的家兔免疫血清对国际上血清Ⅰ型和Ⅱ型参考毒株进行试验,除分离自褐家鼠的K_(24)-株外,其余13株均可定为血清Ⅰ型或血清Ⅱ型用已知的Ⅰ型和Ⅱ型出血热高价免疫血清与K_(24)株进行中和试验,根据两型免疫血清的中和效价则可将K_(24)株定为血清Ⅱ型,但是K_(24)株免疫血清对6株Ⅰ型病毒和4株Ⅱ型病毒的中和效价几乎相同(相差≤2倍),表明K_(24)株是一株具有广谱中和抗原的出血热病毒。
- 流行性出血热病毒株
- , 空斑减少中和试验
- , 血清分型
- , 抗原性分析

Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test

1.
National Institute for the Control of Pharmacentical and Biological Products. Beijing 100050

Abstract

Serotypic classification of 14 HFRS virus isolates from different sources were studied using plaque reduction tests(PRNT) sera of rabbits inoculated with different HFRS viruses were tested against reference type-1 Hantaan virus and type-2 Seoul virus. The results indicated that except one strain(K_(24)), all the 13 isolates could be classified into either serotype 1 or serotype 2 according to the differences(≥4 fold) of the neutralizing titers between the sera reacted against reference type 1 and type 2 vir...
- HFRS virus Plaque reduction neutralization tests Serotyping Antigenic analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test

1. National Institute for the Control of Pharmacentical and Biological Products. Beijing 100050

Abstract: Serotypic classification of 14 HFRS virus isolates from different sources were studied using plaque reduction tests(PRNT) sera of rabbits inoculated with different HFRS viruses were tested against reference type-1 Hantaan virus and type-2 Seoul virus. The results indicated that except one strain(K_(24)), all the 13 isolates could be classified into either serotype 1 or serotype 2 according to the differences(≥4 fold) of the neutralizing titers between the sera reacted against reference type 1 and type 2 vir...