Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins

CHEN Sheng-Min; GUO Hui-Yu

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April 1992

CHEN Sheng-Min and GUO Hui-Yu. Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins[J]. Virologica Sinica, 1992, 7(2).

Citation: CHEN Sheng-Min, GUO Hui-Yu. Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins .VIROLOGICA SINICA, 1992, 7(2) : 166.

应用生物素标记的凝集素分析登革Ⅱ型病毒糖蛋白

陈圣敏 ,
郭辉玉

1.
中山医科大学微生物学与免疫学教研室
2.
中山医科大学微生物学与免疫学教研室广州

摘要

应用十二种生物素标记的凝集素,分析了经SDS-PAGE和电转印分离的登革Ⅱ型病毒(D_2V)糖蛋白。结果表明:D_2V的包膜糖蛋白E可与三种D-甘露糖特异的凝集素(conAPSA及LCA)结合,提示E蛋白的寡糖链为高甘露糖型。D_2V的糖基化非结构蛋白NS_1可与两种D-甘露糖特异的凝集素conA及LCA结合,提示NS_1的寡糖链亦为高甘露糖型。实验结果还显示了一些可能为C6/36细胞感染D_2V后新合成、合成量增加或减少以至完全受抑制的糖蛋白成分,有关这些糖蛋白的性质、功能、意义尚不清楚。在本实验条件下,这种生物素标记凝集素印迹法证明了其敏感性和糖基特异性,可以作为分析各种微量含糖大分子的一种实用方法。
- 凝集素
- , 登革病毒
- , 糖蛋白
- , 印迹技术

Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins

1.
Department of Microbiology and Immunology
2.
Sun Yat-sen University of Medical Sciences

Abstract

In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the infected and mock-infected cell lysates were separated by diseontinous SDS-PAGE and transferred electrophoretically onto NC sheet. The blots were then probed with 12 kinds of biotinylated leetius(Con A, PSA, LCA, SBA, D...
- Biotinylated lectin Lectin blotting Dengue virus Glycoproteins

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins

1. Department of Microbiology and Immunology
2. Sun Yat-sen University of Medical Sciences

Abstract: In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the infected and mock-infected cell lysates were separated by diseontinous SDS-PAGE and transferred electrophoretically onto NC sheet. The blots were then probed with 12 kinds of biotinylated leetius(Con A, PSA, LCA, SBA, D...