Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling

CHEN Jian-Beng; M J Adams

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April 1992

CHEN Jian-Beng and M J Adams. Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling[J]. Virologica Sinica, 1992, 7(2).

Citation: CHEN Jian-Beng, M J Adams. Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling .VIROLOGICA SINICA, 1992, 7(2) : 207.

应用免疫胶体金技术定位感病大麦细胞中的大麦和性花叶病毒

陈剑平 ,
M J Adams

1.
浙江省农业科学院病毒学实验室
2.
英国AFRC洛桑试验站植物病理学系杭州

摘要

本文报道免疫胶体金标记技术的建立,并用此技术定位大麦叶和根组织超薄切片中大麦和性花叶病毒(BaMMV)。在感染病毒的大麦叶和根细胞中,病毒束、游离病毒颗粒和病毒外壳蛋白多分布于细胞质丰富的细胞中,且以液泡和叶绿体(仅叶组织)周围较多。在细胞器已解体的病根表皮细胞中,有时也可检测到大量游离病毒粒子。少数风轮体或板状集结体上也存在病毒或病毒外壳蛋白。细胞核、叶绿体、线粒体、细胞膜以及其他细胞器上都未见有特异性金颗粒标记。
- 免疫胶体金标记
- , 大麦和性花叶病病毒
- , 定位

Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling

1.
1 Zhejiang Academy of Agricultural Sciences
2.
Hangzhou 310021

Abstract

An immuno-colloidal gold labelling procedure was developed to localize barley mild mosaic virus(BaMMV) in thin sections of barley leaf and root tissues embedded in L. R. White resin, The optimal procedure for specific labelling of virus with a minimum of background labelling and economical use of reagents was to treat sections with buffer containing bovine serum albumen to block non-specific binding sites, treat with BaMMV-IgG(1/1000; 15 rain at room temperature), wash and label with goat anti-rabbit IgG-go...
- Immuno-colloidal gold labelling Barley mild mosaic virus Localization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling

1. 1 Zhejiang Academy of Agricultural Sciences
2. Hangzhou 310021

Abstract: An immuno-colloidal gold labelling procedure was developed to localize barley mild mosaic virus(BaMMV) in thin sections of barley leaf and root tissues embedded in L. R. White resin, The optimal procedure for specific labelling of virus with a minimum of background labelling and economical use of reagents was to treat sections with buffer containing bovine serum albumen to block non-specific binding sites, treat with BaMMV-IgG(1/1000; 15 rain at room temperature), wash and label with goat anti-rabbit IgG-go...