The Viral Inclusion Bodies in the Autopsy Tissues of Patients with Epidemic Hemorrhagic Fever

YANG Shou-Jing; LIU Yan-Fang; HAN Yan-Ping; XU Zhi-Kai; YAN Pei-Song

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June 1992

YANG Shou-Jing, LIU Yan-Fang, HAN Yan-Ping, XU Zhi-Kai and YAN Pei-Song. The Viral Inclusion Bodies in the Autopsy Tissues of Patients with Epidemic Hemorrhagic Fever[J]. Virologica Sinica, 1992, 7(3).

Citation: YANG Shou-Jing, LIU Yan-Fang, HAN Yan-Ping, XU Zhi-Kai, YAN Pei-Song. The Viral Inclusion Bodies in the Autopsy Tissues of Patients with Epidemic Hemorrhagic Fever .VIROLOGICA SINICA, 1992, 7(3) : 246.

流行性出血热病人尸检材料中的病毒包涵体

1.
第四军医大学病理教研室
2.
第四军医大学病理教研室西安

摘要

应用抗流行性出血热病毒多克隆及单克隆抗体结合敏感的方法在尸检组织切片上进行了免疫细胞化学染色,多克隆抗体可清楚地显示出尸检组织中病毒抗原阳性的包涵体结构。依光镜下染色的形态特点不同,可将包涵体分为四个类型即小泡型、大泡型、多泡型和实心型。单克隆抗体染色进一步证实,实心型及空泡型包涵体呈“N”抗原阳性,少数组织中空泡状包涵体也呈“G2”抗原阳性。病毒包涵体的出现可作为该病毒在人体细胞内感染及复制的形态学证据。
- 出血热流行性
- , 尸检
- , 病毒包涵体
- , 免疫细胞化学

The Viral Inclusion Bodies in the Autopsy Tissues of Patients with Epidemic Hemorrhagic Fever

1.
Department of Pathology
2.
Fourth Military Medical College

Abstract

Using polyclonal antibody (PAb)and monoclonal antibodies(MAbs) against epidemic hemorrhagic fever virus (EHFV), the viral antigens in fresh and long-term storage EHF autopsy tissues were studied comparatively with more sensitive multi-layer PAP and PAP combined with ABC (PAP+ABC) techniques. The results showed that the inclusion bodies (IB) with viral antigens positive could be demonstrated in the autopsy tissues. Four types of IB, designated solid. small-bubble, big-bubble and multi-bubble, could be observ...
- Hemorrhagic Fever Epidemic Autopsy Immunocytochemistry Viral Inclusion Bodies

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Viral Inclusion Bodies in the Autopsy Tissues of Patients with Epidemic Hemorrhagic Fever

1. Department of Pathology
2. Fourth Military Medical College

Abstract: Using polyclonal antibody (PAb)and monoclonal antibodies(MAbs) against epidemic hemorrhagic fever virus (EHFV), the viral antigens in fresh and long-term storage EHF autopsy tissues were studied comparatively with more sensitive multi-layer PAP and PAP combined with ABC (PAP+ABC) techniques. The results showed that the inclusion bodies (IB) with viral antigens positive could be demonstrated in the autopsy tissues. Four types of IB, designated solid. small-bubble, big-bubble and multi-bubble, could be observ...