Studies on the Viral Polypeptides and Serological Relationships of Four Strains of Rabbit Haemorrhagic Disease Virus (RHDV)

SHU Wen; LUO Jing; YANG Hua-Lou

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June 1992

SHU Wen, LUO Jing and YANG Hua-Lou. Studies on the Viral Polypeptides and Serological Relationships of Four Strains of Rabbit Haemorrhagic Disease Virus (RHDV)[J]. Virologica Sinica, 1992, 7(3).

Citation: SHU Wen, LUO Jing, YANG Hua-Lou. Studies on the Viral Polypeptides and Serological Relationships of Four Strains of Rabbit Haemorrhagic Disease Virus (RHDV) .VIROLOGICA SINICA, 1992, 7(3) : 334.

兔出血症病毒四个分离株的病毒多肽和血清学关系研究

1.
成都四川大学生物工程系
2.
中国科学院武汉病毒研究所
3.
中国科学院武汉病毒研究所武汉

摘要

使用PEG-DS两相系统和超离心提纯的兔出血症病毒(RHDV)四个分离株病毒,再经Sepharose 4B柱层析进一步提纯后,得到较纯的病毒粒子,回收率可达70％以上。应用常规双向免疫扩散试验,交叉血凝抑制试验和酶联免疫吸附试验(ELISA)对四个不同地区分离株间的血清学关系进行了比较研究。结果表明实验中的四个分离株病毒均属同一血清型。SDS-PAGE结果表明,这四个分离株病毒均含有四条多肽,分子量为28—64KD。各株病毒多肽的分子量和各多肽在病毒粒子总蛋白中所占比例略有差异。因此四个分离株的RHDV在蛋白结构上可能存在地区差异。
- 兔出血症病毒
- , 血清学关系
- , 病毒多肽

Studies on the Viral Polypeptides and Serological Relationships of Four Strains of Rabbit Haemorrhagic Disease Virus (RHDV)

1.
Wuhan lnstitute of Virology
2.
Academia Sinica
3.
Wuhan 430071

Abstract

In this paper four different strains of RHDV were purified by two phases of polyethylene glyeol-dextran sodium sulfate andcentrifugation, then virions in highly pure form were obtained by using Sepharose 4B chromatography. The recovery rate of virus preparation was about 70 pereent. Serological relationships of four different strains of RHDV were studied by using immuno double diffusion, cross hemagglutination inhibition and enzyme-linked immunosorbent assay(ELISA). The results showed that serotypes are the...
- Rabbit haemorrhagic disease virus Serological relationship Viral polypeptide

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on the Viral Polypeptides and Serological Relationships of Four Strains of Rabbit Haemorrhagic Disease Virus (RHDV)

1. Wuhan lnstitute of Virology
2. Academia Sinica
3. Wuhan 430071

Abstract: In this paper four different strains of RHDV were purified by two phases of polyethylene glyeol-dextran sodium sulfate andcentrifugation, then virions in highly pure form were obtained by using Sepharose 4B chromatography. The recovery rate of virus preparation was about 70 pereent. Serological relationships of four different strains of RHDV were studied by using immuno double diffusion, cross hemagglutination inhibition and enzyme-linked immunosorbent assay(ELISA). The results showed that serotypes are the...