Studies on Replication of Rabbit Hemorrhagic Disease Virus (RHDV) in Vitro

LUO Jing; YANG Hua-Lou; LIU Gong; YAN Yin-Fang; WANG Han-Zhong; SUN Song-Bai

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June 1992

LUO Jing, YANG Hua-Lou, LIU Gong, YAN Yin-Fang, WANG Han-Zhong and SUN Song-Bai. Studies on Replication of Rabbit Hemorrhagic Disease Virus (RHDV) in Vitro[J]. Virologica Sinica, 1992, 7(3).

Citation: LUO Jing, YANG Hua-Lou, LIU Gong, YAN Yin-Fang, WANG Han-Zhong, SUN Song-Bai. Studies on Replication of Rabbit Hemorrhagic Disease Virus (RHDV) in Vitro .VIROLOGICA SINICA, 1992, 7(3) : 343.

兔出血症病毒体外复制的研究

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

在几种动物细胞上,采用同步感染的方法研究了兔出血症病毒(RHDV)的复制特性。病毒感染细胞后(PI)48—72小时可观察到明显的细胞病变,血凝效价可增高5—10倍。病毒对细胞传代代数不同,敏感性也不同。在兔婴肾(RK)和兔婴肺(RL)细胞上以4—8代最为敏感。采用免疫荧光染色法,经病毒感染48—72小时的细胞中可观察到特异性荧光。细胞增殖的病毒经PEG-DS浓缩,Sepharose 4B柱层析提纯后,在电镜下可观察到完整的病毒粒子,将此病毒回接健康实验兔可致100％死亡。免疫双扩散和免疫电泳试验表明,细胞增殖的病毒抗原与来自病兔肝的RHDV抗血清之间产生明显的沉淀带。SDS-PAGE分析病毒获得四条多肽,其分子量大小与病兔肝组织提取的病毒蛋白多肽分子量相比略有差异。
- 兔出血症病毒
- , 体外复制
- , 血凝效价
- , 病毒多肽

Studies on Replication of Rabbit Hemorrhagic Disease Virus (RHDV) in Vitro

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

In this paper the characteristics of replication of RHDV in the RL and RK cells have been studied by using synchronous infective method. The cytopathology effect can be observed at 48—96 hrs after viruses infection. HA titer of viral increased 5—10 times as high as those HA titers of viruses infection at 72 hr. The sensitivity of RHDV to passage cells are different, it is most sensitive for 4—8 generation. Special fluorescence in the ceils can be seen at 18—72 hrs using immunofluorescence stain. Viruses of ...
- Rabbit hemorrhagic disease Replication in vitro HA titer Virus polypeptide

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Replication of Rabbit Hemorrhagic Disease Virus (RHDV) in Vitro

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: In this paper the characteristics of replication of RHDV in the RL and RK cells have been studied by using synchronous infective method. The cytopathology effect can be observed at 48—96 hrs after viruses infection. HA titer of viral increased 5—10 times as high as those HA titers of viruses infection at 72 hr. The sensitivity of RHDV to passage cells are different, it is most sensitive for 4—8 generation. Special fluorescence in the ceils can be seen at 18—72 hrs using immunofluorescence stain. Viruses of ...