Expression of Hepatitis B Virus Surface Antigen Using a Baculovirus Vector with the Polyhedrin Gene

HE Dai-Fen; WANG Xun-Zhang; XIE Wei-Dong; CHEN Ru; LONG Qi-Xin; DENG Ri-Jiang; BO Zhe-Long; SU De-Meng

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August 1992

HE Dai-Fen, WANG Xun-Zhang, XIE Wei-Dong, CHEN Ru, LONG Qi-Xin, DENG Ri-Jiang, BO Zhe-Long and SU De-Meng. Expression of Hepatitis B Virus Surface Antigen Using a Baculovirus Vector with the Polyhedrin Gene[J]. Virologica Sinica, 1992, 7(4).

Citation: HE Dai-Fen, WANG Xun-Zhang, XIE Wei-Dong, CHEN Ru, LONG Qi-Xin, DENG Ri-Jiang, BO Zhe-Long, SU De-Meng. Expression of Hepatitis B Virus Surface Antigen Using a Baculovirus Vector with the Polyhedrin Gene .VIROLOGICA SINICA, 1992, 7(4) : 389.

表达乙型肝炎病毒表面抗原基因又形成多角体的杆状病毒

1.
中山大学昆虫学研究所、生物防治国家重点实验室
2.
复旦大学病毒学研究室广州

摘要

用形成包含体(OCC~+)并能利用人工合成启动序列和多角体XIV启动子表达外源基因的转移载体质粒pSXIVVI~+X3,将乙型肝炎病毒表面抗原(HBsAg)基因和多角体基因同时插入无包含体的粉纹夜蛾核型多角体病毒TnNPV-SVI-G基因组中,得到表达HBsAg基因又形成包含体(多角体)的重组毒侏TnNPV-HBs85-OCC~+。与利用野生型多角体启动子表达HBsAg基因的无包含体毒株TnNPV-HBsD4不同,TnNPV-HBs85-OCC~+由于具包含体,能以口服方式大规模感染粉纹夜蛾(Trichoplusia ni)幼虫,且HBsAg基因在草地夜蛾(Spodoptera frugiperda)离体细胞中的表达量要比前者高约37％,在虫体中的表达则更高。
- 乙型肝炎病毒表面抗原基因
- , 多角体基因
- , 人工合成启动子
- , 粉纹夜蛾
- , 草地夜蛾细胞

Expression of Hepatitis B Virus Surface Antigen Using a Baculovirus Vector with the Polyhedrin Gene

1.
Institute of Entomology
2.
Zhongshan University and National Laboratory of Biological Control

Abstract

Using a polyhedrin-positive transfer plasmid and a nonoccluded (OCC-) parental recombinant, an occluded (OCC~+)recombinant baculovirusdesignated TnNPV-HBs85-OCC~+ was successfully constructed which contained the gene coding for the hepatitis B virus surface antigen under the dual control of the synthetic and XIV promoters, Infection of Tvichoplusia ni larvae with TnNPVHBs85-OCC~+ can be introduced as a contaminant of the diet rather than the conventional baculovirus OCC~- expression vector in which the reco...
- Hepatitis B virus surface antigen
- , Polyhedrin
- , synthetic promoter
- , Trichoplusia ni Spodoptera frugiperda cells

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Hepatitis B Virus Surface Antigen Using a Baculovirus Vector with the Polyhedrin Gene

1. Institute of Entomology
2. Zhongshan University and National Laboratory of Biological Control

Abstract: Using a polyhedrin-positive transfer plasmid and a nonoccluded (OCC-) parental recombinant, an occluded (OCC~+)recombinant baculovirusdesignated TnNPV-HBs85-OCC~+ was successfully constructed which contained the gene coding for the hepatitis B virus surface antigen under the dual control of the synthetic and XIV promoters, Infection of Tvichoplusia ni larvae with TnNPVHBs85-OCC~+ can be introduced as a contaminant of the diet rather than the conventional baculovirus OCC~- expression vector in which the reco...