Morphology and Biochemical Charateristics of the Nuclear Polyhedrosis Virus of Malacosoma neustria testacea

ZHANG Wen-Jun; CHEN Dai-Hua; ZHANG Li-Ren; ZHANG Guo-Xian; LI Shu-Qin

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August 1992

ZHANG Wen-Jun, CHEN Dai-Hua, ZHANG Li-Ren, ZHANG Guo-Xian and LI Shu-Qin. Morphology and Biochemical Charateristics of the Nuclear Polyhedrosis Virus of Malacosoma neustria testacea[J]. Virologica Sinica, 1992, 7(4).

Citation: ZHANG Wen-Jun, CHEN Dai-Hua, ZHANG Li-Ren, ZHANG Guo-Xian, LI Shu-Qin. Morphology and Biochemical Charateristics of the Nuclear Polyhedrosis Virus of Malacosoma neustria testacea .VIROLOGICA SINICA, 1992, 7(4) : 417.

黄褐天幕毛虫核型多角体病毒的形态结构与某些生化特性的研究

1.
中国科学院武汉病毒研究所
2.
内蒙古农牧学院农学系
3.
内蒙古园艺科学研究所武汉
4.
呼和浩特

摘要

黄褐天幕毛虫核型多角体病毒是一种多粒包埋型病毒。在扫描电镜下观察,多角体呈不规则多面体,大小不一致,平均直径为1.30μm。病毒粒子为杆状,大小为366×90nm。病毒核酸为一环状DNA,长度约为37.2μm。经限制性内切酶酶解分析,DNA总分子量为77.1×10~6D。病毒多角体蛋白宫含ASP和Glu,而His、Cys、Met含量较少。经SDS-PAGE分析,多角体蛋白主带的分子量为30.2KD;病毒粒子含21条多肽,分子量范围在16.5—107KD之间。
- 黄褐天幕毛虫
- , 核型多角体病毒
- , 多肽分析
- , DNA酶切图谱
- , 亚克分子带

Morphology and Biochemical Charateristics of the Nuclear Polyhedrosis Virus of Malacosoma neustria testacea

1.
Wuhan Institute of Virology
2.
Academia Siuiea
3.
Wuhan 130071Inner Mongolia College of Agriculture & Auimal Husbandry
4.
Huhehaote

Abstract

Malacosoma neustria testacea nuclear polyhedrosis virus(MntNPV)is a kind of multi-embeded baculovirus, Scanning EM shows that MntNPV inclusion bodies are irreguIar polyhedra with different sizes The average diameter of polyhedra is 1.3μm. Virions have a mean size of 366×90nm, The virus genome is a circular DNA macromolecule with length of 37.2μm. SDS-PAGE exhibits that polyhcdrin has only oue major polypeptide with MW of 30.2KD, Virions have at least 21 polypeptide bands with MW ranged from 16.5KD to 107KD....
- Malacosoma neustria testacea
- , Nuclear Polyhedrosis Virus Amino Acid Composition Analysis
- , Restriction Endonuclease Map
- , Submolecular Fragment

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Morphology and Biochemical Charateristics of the Nuclear Polyhedrosis Virus of Malacosoma neustria testacea

1. Wuhan Institute of Virology
2. Academia Siuiea
3. Wuhan 130071Inner Mongolia College of Agriculture & Auimal Husbandry
4. Huhehaote

Abstract: Malacosoma neustria testacea nuclear polyhedrosis virus(MntNPV)is a kind of multi-embeded baculovirus, Scanning EM shows that MntNPV inclusion bodies are irreguIar polyhedra with different sizes The average diameter of polyhedra is 1.3μm. Virions have a mean size of 366×90nm, The virus genome is a circular DNA macromolecule with length of 37.2μm. SDS-PAGE exhibits that polyhcdrin has only oue major polypeptide with MW of 30.2KD, Virions have at least 21 polypeptide bands with MW ranged from 16.5KD to 107KD....