Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

PENG Hai; CHEN Xue-Ren; WU Jian-Hua; GONG Jie-Xun

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August 1992

PENG Hai, CHEN Xue-Ren, WU Jian-Hua and GONG Jie-Xun. Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA[J]. Virologica Sinica, 1992, 7(4).

Citation: PENG Hai, CHEN Xue-Ren, WU Jian-Hua, GONG Jie-Xun. Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA .VIROLOGICA SINICA, 1992, 7(4) : 426.

烟草花叶病毒装配起始位点反义RNA表达型中间载体的构建

1.
中国科学院上海生物化学研究所
2.
中国科学院上海生物化学研究所上海 20003
3.
上海 20003

摘要

利用基因工程方法培育抗病毒植物新品种的途径之一,是在植株中建立一个产生病毒基因组功能片段的反义RNA的系统。本工作设计并合成了一段烟草花叶病毒(TMV)装配起始位点反义RNA的基因,再以pBR 325为基本质粒,构建了包含带有花椰菜花叶病毒(CaMV)35S启动子和PolyA信号的反义RNA表达单元,以及为筛选转基因植株所必须的NPT-Ⅱ表达单元的中间载体,为以后经土壤农杆菌而获得转基因烟草植株打下了基础。
- 烟草花叶病毒（TMV）
- , 反义RNA
- , 中间载体

Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

1.
Shanghai Institute of Biochemistry
2.
Academia Sinica
3.
Shanghai 200031

Abstract

In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.
- Tobacco mosaic virus （TMV）
- , Antisense RNA
- , Intermediate vector

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

1. Shanghai Institute of Biochemistry
2. Academia Sinica
3. Shanghai 200031

Abstract: In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.